Reactome | Palmitoylation of eNOS

Palmitoylation of eNOS

Stable Identifier

R-HSA-203567

Type

Reaction [transition]

Species

Homo sapiens

Compartment

Golgi membrane

Synonyms

hexadecanoyl-CoA + L-cysteinyl-(protein) => CoA + S-hexadecanoyl-L-cysteinyl-(protein)

ReviewStatus

5/5

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ZDHHC21, associated with the Golgi membrane, palmitoylates cytosolic eNOS (Nitric oxide synthase 3), enabling its localization to plasmalemmal caveolae (Garcia-Cardena et al. 1996; Shaul et al. 1996). Localization to this microdomain is likely to optimize eNOS activation and the extracellular release of nitric oxide. ZDHHC21 belongs to a family of DHHC domain-containing cysteine-rich proteins, several of which can mediate eNOS palmitoylation in cultured human endothelial cells. Only inhibition of ZDHHC21 palmitoyl transferase reduces eNOS palmitoylation, eNOS targeting, and stimulated NO production (Fernández-Hernando et al. 2006), so only ZDHHC21 activity is annotated here.

Literature References

PubMed ID	Title	Journal	Year
16864653	Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase Sessa, WC, Bredt, DS, Bernatchez, PN, Fernandez-Hernando, C, Fukata, M, Lin, MI, Fukata, Y	J Cell Biol	2006
8626455	Acylation targets emdothelial nitric-oxide synthase to plasmalemmal caveolae Michel, T, Robinson, LJ, Yuhanna, IS, Shaul, PW, German, Z, Smart, EJ, Anderson, RG, Ying, Y	J Biol Chem	1996
8692835	Targeting of nitric oxide synthase to endothelial cell caveolae via palmitoylation: implications for nitric oxide signaling Schnitzer, JE, Liu, J, Sessa, WC, Garcia-Cardena, G, Oh, P	Proc Natl Acad Sci U S A	1996