NMNAT3 transfers an adenylyl group from ATP to NAMN to yield NAAD

Stable Identifier
R-HSA-200474
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
Nicotinate D-ribonucleotide + ATP => deamino-NAD+ + pyrophosphate [NMNAT3]
Locations in the PathwayBrowser
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NMNAT3 catalyzes the reaction of nicotinate D-ribonucleotide and ATP to form deamino-NAD+ (nicotinate adenine dinucleotide) and pyrophosphate (Sorci et al. 2007). The active form of the enzyme is a tetramer in vitro (Zhang et al. 2003). Recombinant FLAG-tagged protein expressed in HeLa cells localizes both to the cytosol and to mitochondria (Berger et al. 2005). The cytosolic protein is annotated here.

Literature References
PubMed ID Title Journal Year
17402747 Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis

Sorci, L, Cimadamore, F, Scotti, S, Petrelli, R, Cappellacci, L, Franchetti, P, Orsomando, G, Magni, G

Biochemistry 2007
12574164 Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis

Zhang, X, Kurnasov, OV, Karthikeyan, S, Grishin, NV, Osterman, AL, Zhang, H

J Biol Chem 2003
16118205 Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms

Berger, F, Lau, C, Dahlmann, M, Ziegler, M

J Biol Chem 2005
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
nicotinate-nucleotide adenylyltransferase activity of 4xNMNAT3:4xMg2+ [mitochondrial matrix]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Authored
Created