Cytosolic MDH1 (malate dehydrogenase 1) catalyzes the reaction of malate (MAL) and NAD+ to form oxaloacetate (OA) and NADH + H+ (Friedrich et al. 1988; Lo et al. 2005). The dimeric structure of the human dehydrogenase is inferred from that established for its well-studied pig homolog (Birktoft et al. 1989).
L-malate dehydrogenase activity of MDH1 dimer [cytosol]