NAD synthases 1 and 2 (NADSYN1 and NADSYN2) catalyse the final step in the biosynthesis of NAD+, both in de novo synthesis and in the salvage pathway. The enzymes makes use of an amide donor in the reaction. NADSYNs exist as a homohexamers in the cytosol. The major difference between the two forms is that NADSYN1 appears to be glutamine-dependent whereas NADSYN2 is strictly ammonia-dependent (Hara et al. 2003).
Yamada, K, Terashima, M, Shimoyama, M, Osago, H, Hara, N, Tsuchiya, M
NAD+ synthase (glutamine-hydrolyzing) activity of NADSYN1 hexamer [cytosol]
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