CHPF,CHSY3 transfer GalNAc to chondroitin

Stable Identifier
Reaction [transition]
Homo sapiens
GalNAc is added to chondroitin
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An N-acetylgalactosamine (GalNAc) moiety is added to the chondroitin chain by dual-activity enzymes, the chondroitin sulfate synthases 1-3 (CHSY1, CHPF and CHSY3 respectively) (Kitagawa et al. 2001, Yada et al. 2003, Yada et al. 2003b). They possess both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity, the latter activity used in this reaction. These three enzymes require divalent metals as cofactors, manganese producing the highest activities. The repeated disaccharide units of GlcA-GalNAc identify this glycosaminoglycan as chondroitin.

Literature References
PubMed ID Title Journal Year
11514575 Molecular cloning and expression of a human chondroitin synthase

Uyama, T, Kitagawa, H, Sugahara, K

J Biol Chem 2001
12761225 Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities

Yada, T, Kwon, YD, Kudo, T, Iwasaki, H, Gotoh, M, Watanabe, H, Go, M, Togayachi, A, Kimata, K, Sato, T, Kaseyama, H, Shionyu, M, Narimatsu, H, Kikuchi, N

J Biol Chem 2003
12907687 Chondroitin sulfate synthase-3. Molecular cloning and characterization

Yada, T, Kwon, YD, Sato, T, Kudo, T, Iwasaki, H, Gotoh, M, Watanabe, H, Kaseyama, H, Narimatsu, H, Togayachi, A, Kikuchi, N, Kimata, K

J Biol Chem 2003
Catalyst Activity

glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity of CHPF,CHSY3 [Golgi membrane]

Orthologous Events
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