Reactome | 2xTPK1:Mg2+ phosphorylates THMN

2xTPK1:Mg2+ phosphorylates THMN

Stable Identifier

R-HSA-196761

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

Thiamin is pyrophosphorylated

ReviewStatus

5/5

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Cytosolic thiamin pyrophosphokinase (TPK1) catalyzes the reaction of thiamin (THMN) and ATP to form thiamin diphosphate (ThDP aka thiamin pyrophosphate) and ADP. ThDP is an active cofactor for transketolase, pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase, enzymes involved in glycolysis and energy production. The gene encoding the human enzyme has been cloned and its protein product has been shown to have TPK1 activity (Nosaka et al. 2001; Zhao et al. 2001). Its homodomeric structure and association with Mg2+ are inferred from properties of the homologous yeast enzyme (Baker et al. 2001).

Literature References

PubMed ID	Title	Journal	Year
11342117	Molecular cloning of human thiamin pyrophosphokinase Zhao, R, Gao, F, Goldman, ID	Biochim Biophys Acta	2001
11342111	Isolation and characterization of a human thiamine pyrophosphokinase cDNA Nosaka, K, Onozuka, M, Kakazu, N, Hibi, S, Nishimura, H, Nishino, H, Abe, T	Biochim Biophys Acta	2001