2xTPK1:Mg2+ phosphorylates THMN

Stable Identifier
Reaction [transition]
Homo sapiens
Thiamin is pyrophosphorylated
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Cytosolic thiamin pyrophosphokinase (TPK1) catalyzes the reaction of thiamin (THMN) and ATP to form thiamin diphosphate (ThDP aka thiamin pyrophosphate) and ADP. ThDP is an active cofactor for transketolase, pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase, enzymes involved in glycolysis and energy production. The gene encoding the human enzyme has been cloned and its protein product has been shown to have TPK1 activity (Nosaka et al. 2001; Zhao et al. 2001). Its homodomeric structure and association with Mg2+ are inferred from properties of the homologous yeast enzyme (Baker et al. 2001).

Literature References
PubMed ID Title Journal Year
11342117 Molecular cloning of human thiamin pyrophosphokinase

Goldman, ID, Gao, F, Zhao, R

Biochim Biophys Acta 2001
11342111 Isolation and characterization of a human thiamine pyrophosphokinase cDNA

Nosaka, K, Hibi, S, Nishimura, H, Abe, T, Nishino, H, Kakazu, N, Onozuka, M

Biochim Biophys Acta 2001
Catalyst Activity

thiamine diphosphokinase activity of 2xTPK1:Mg2+ [cytosol]

Orthologous Events
Cross References
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