RNA polymerase III transcribes microbial dsDNA to dsRNA

Stable Identifier
Reaction [omitted]
Homo sapiens
Related Species
Human gammaherpesvirus 4, Human herpesvirus 1, Legionella pneumophila
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RNA polymerase III (POL III) was reported to sense and transcribe cytosolic AT-rich dsDNA into 5'-triphosphate poly(A-U) RNA in human and mouse cells. This dsRNA ligand in turn activated retinoic acid-inducible gene I (RIG-I) leading to production of type I interferon and activation of the transcription factor NF-kappaB (Chiu YH et al. 2009, Ablasser A et al. 2009). Knockdown of POL III expression by siRNA or inhibition of its enzymatic activity by specific chemical inhibitor ML-60218 prevented IFN beta induction in HEK293 cells stimulated with DNA viruses or poly(dA-dT) (Chiu YH et al. 2009, Ablasser A et al. 2009). Moreover, Pol-III inhibition blocked interferon induction by intracellular Legionella pneumophila bacteria [Chiu YH et al 2009].

This project represents cytosolic RNA polymerase III as a complex comprising 17 subunits, although the precise biochemical composition of the cytosolic holoenzyme complex which specifically recognizes AT-rich DNA is not yet known.

Literature References
PubMed ID Title Journal Year
19631370 RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway

Chiu, YH, Chen, ZJ, Macmillan, JB

Cell 2009
19609254 RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate

Fitzgerald, KA, Ablasser, A, Bauernfeind, FG, Hornung, V, Latz, E, Hartmann, G

Nat Immunol 2009
Catalyst Activity

RNA polymerase III activity of RNA Polymerase III Holoenzyme [cytosol]

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