Reactome | Pregnenolone is dehydrogenated to form pregn-5-ene-3,20-dione

Pregnenolone is dehydrogenated to form pregn-5-ene-3,20-dione

Stable Identifier

R-HSA-196350

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, endoplasmic reticulum membrane

Synonyms

Pregnenolone + NAD+ => pregn-5-ene-3,20-dione + NADH + H+

ReviewStatus

5/5

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Pregnenolone is dehydrogenated to form pregn-5-ene-3,20-dione

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Pregnenolone and NAD+ react to form pregn-5-ene-3,20-dione and NADH + H+. This reaction is catalyzed by the 3 beta-hydroxysteroid activity of 3-beta-hydroxysteroid dehydrogenase/isomerase (HSD3B) enzyme associated with the endoplasmic reticulum membrane. The active form of the enzyme is a homodimer. The enzyme occurs in two isoforms that are similar in their biochemical properties but differ in their tissue expression: type I (HSD3B1) is found in placenta and skin, while type II (HSD3B2) is found in the adrenal glands and gonads.

Literature References

PubMed ID	Title	Journal	Year
2770297	Human placental 3 beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5----4-ene-isomerase: purification from mitochondria and kinetic profiles, biophysical characterization of the purified mitochondrial and microsomal enzymes Thomas, JL, Myers, RP, Strickler, RC	J Steroid Biochem	1989
1944309	Structure and expression of a new complementary DNA encoding the almost exclusive 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase in human adrenals and gonads Rheaume, E, Lachance, Y, Zhao, HF, Breton, N, Dumont, M, de Launoit, Y, Trudel, C, Luu-The, V, Simard, J, Labrie, F	Mol Endocrinol	1991
12832414	Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase Thomas, JL, Duax, WL, Addlagatta, A, Brandt, S, Fuller, RR, Norris, W	J Biol Chem	2003
2243100	Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells Lachance, Y, Luu-The, V, Labrie, C, Simard, J, Dumont, M, de Launoit, Y, Guerin, S, Leblanc, G, Labrie, F	J Biol Chem	1990