botA LC cleaves target cell SNAP25

Stable Identifier
R-HSA-194818
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
cytosol, plasma membrane
ReviewStatus
5/5
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botA LC cleaves target cell SNAP25
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Botulinum toxin type A light chain (botA LC), in the cytosol of a target cell, catalyzes the removal of a carboxyterminal peptide from synaptosomal-associated protein 25 (SNAP25). botA LC is a zinc metalloprotease (Binz et al. 1994; Schiavo et al. 1993). SNAP25 is associated with the cytosolic face of the target cell plasma membrane where it forms part of a complex required for synaptic vesicle docking and exocytosis. Its cleavage by botulinum toxin blocks synaptic vesicle fusion with the plasma membrane and neurotransmitter release and in vivo leads to a long lasting flaccid paralysis (Sudhof et al, 1993; Sudhof 2004).
Literature References
PubMed ID Title Journal Year
8402889 Membrane fusion machinery: insights from synaptic proteins

Südhof, TC, Niemann, H, Jahn, R, De Camilli, P

Cell 1993
8243676 Botulinum neurotoxins serotypes A and E cleave SNAP-25 at distinct COOH-terminal peptide bonds

Mehta, PP, Schiavo, G, DasGupta, BR, Montecucco, C, Wilson, MC, Santucci, A, Jontes, J, Benfenati, F

FEBS Lett 1993
15217342 The synaptic vesicle cycle

Südhof, TC

Annu Rev Neurosci 2004
8294407 Proteolysis of SNAP-25 by types E and A botulinal neurotoxins

Südhof, TC, Link, E, Yamasaki, S, Baumeister, A, Niemann, H, Binz, T, Jahn, R, Blasi, J

J Biol Chem 1994
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Output
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as an event of
Catalyst Activity

metalloendopeptidase activity of botA LC:Zn2+ [cytosol]

Physical Entity
botA LC:Zn2+ [cytosol] (Clostridium botulinum)
Activity
metalloendopeptidase activity (GO:0004222)
Disease
Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Authored
Gopinathrao, G  (2006-06-15)
Krupa, S  (2006-06-15)
Reviewed
Ichtchenko, K  (2007-08-03)
Thirunavukkarasu, N  (2014-11-18)
Sharma, S  (2014-11-18)
Created
Gopinathrao, G  (2007-03-27)
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