CYP8B1 12-hydroxylates 4CHOL7a,27DONE

Stable Identifier
R-HSA-193845
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
4-Cholesten-7alpha,27-diol-3-one is 12alpha-hydroxylated to 4-Cholesten-7alpha,12alpha,27-triol-3-one, 4-Cholesten-7alpha,27-diol-3-one + NADPH + H+ + O2 => 4-Cholesten-7alpha,12alpha,27-triol-3-one + NADP+ + H2O
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Sterol 12alpha hydroxylase (CYP8B1), associated with the endoplasmic reticulum membrane, catalyses the 12-alpha-hydroxylation of 4-Cholesten-7alpha,27-diol-3-one (4CHOL7a,27DONE) to 4-Cholesten-7alpha,12alpha,27-triol-3-one. While the human gene has been cloned (Gafvels et al. 1999), its protein product has not been characterised, and the enzymatic properties of human CYP8B1 protein are inferred from those of its well-characterised rabbit homolog (Ishida et al. 1992).

Literature References
PubMed ID Title Journal Year
10051404 Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns

Gafvels, M, Olin, M, Chowdhary, BP, Raudsepp, T, Andersson, U, Persson, B, Jansson, M, Bjorkhem, I, Eggertsen, G

Genomics 1999
1400444 Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase

Ishida, H, Noshiro, M, Okuda, K, Coon, MJ

J. Biol. Chem. 1992
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity of Cytochrome P450 (CYP8B1 based) [endoplasmic reticulum membrane]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created
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