Reactome | CYP8B1 12-hydroxylates 4CHOL7a,24(S)DIOL

CYP8B1 12-hydroxylates 4CHOL7a,24(S)DIOL

Stable Identifier

R-HSA-193709

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, endoplasmic reticulum membrane

Synonyms

4-Cholesten-7alpha,24(S)-diol-3-one is 12alpha-hydroxylated to 4-cholesten-7-alpha,12-alpha,24(S)-triol-3-one, 4-Cholesten-7alpha,24(S)-diol-3-one + NADPH + H+ + O2 => 4-cholesten-7-alpha,12-alpha,24(S)-triol-3-one + NADP+ + H2O

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Metabolism (Homo sapiens)

- Biological oxidations (Homo sapiens)
  - Phase I - Functionalization of compounds (Homo sapiens)
    - Cytochrome P450 - arranged by substrate type (Homo sapiens)
      - Endogenous sterols (Homo sapiens)
        
        Sterols are 12-hydroxylated by CYP8B1 (Homo sapiens)
        
        CYP8B1 12-hydroxylates 4CHOL7a,24(S)DIOL (Homo sapiens)
- Metabolism of lipids (Homo sapiens)
  - Metabolism of steroids (Homo sapiens)
    - Bile acid and bile salt metabolism (Homo sapiens)
      - Synthesis of bile acids and bile salts (Homo sapiens)
        
        Synthesis of bile acids and bile salts via 24-hydroxycholesterol (Homo sapiens)
        
        CYP8B1 12-hydroxylates 4CHOL7a,24(S)DIOL (Homo sapiens)

General

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CYP8B1 12-hydroxylates 4CHOL7a,24(S)DIOL

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Sterol 12alpha hydroxylase (CYP8B1) catalyses the 12-alpha-hydroxylation of 4-cholesten-7alpha,24(S)-diol-3-one (4CHOL7a,24(S)DONE) to 4-cholesten-7-alpha,12-alpha,24(S)-triol-3-one (4CHOL7a,12a,24(S)TONE). While the human gene has been cloned (Gafvels et al. 1999), its protein product has not been characterised, and the enzymatic properties of human CYP8B1 protein are inferred from those of its well-characterised rabbit homolog (Ishida et al. 1992).

Literature References

PubMed ID	Title	Journal	Year
1400444	Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase Coon, MJ, Ishida, H, Okuda, K, Noshiro, M	J. Biol. Chem.	1992
10051404	Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns Gafvels, M, Bjorkhem, I, Jansson, M, Raudsepp, T, Chowdhary, BP, Andersson, U, Persson, B, Eggertsen, G, Olin, M	Genomics	1999