Reactome | CYP8B1 12-hydroxylates 4CHOL7aOLONE

CYP8B1 12-hydroxylates 4CHOL7aOLONE

Stable Identifier

R-HSA-192157

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, endoplasmic reticulum membrane

Synonyms

7alpha-hydroxycholest-4-en-3-one + O2 + reduced (NADPH--hemoprotein reductase) => 7alpha,12alpha-dihydroxycholest-4-en-3-one + H(+) + H2O + oxidized (NADPH--hemoprotein reductase), 4-Cholesten-7alpha-ol-3-one is 12alpha-hydroxylated to 7-alpha,12-alpha-dihydroxycholest-4-en-3-one, 7-alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 => 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O

ReviewStatus

5/5

Locations in the PathwayBrowser

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Metabolism (Homo sapiens)

- Biological oxidations (Homo sapiens)
  - Phase I - Functionalization of compounds (Homo sapiens)
    - Cytochrome P450 - arranged by substrate type (Homo sapiens)
      - Endogenous sterols (Homo sapiens)
        
        Sterols are 12-hydroxylated by CYP8B1 (Homo sapiens)
        
        CYP8B1 12-hydroxylates 4CHOL7aOLONE (Homo sapiens)
- Metabolism of lipids (Homo sapiens)
  - Metabolism of steroids (Homo sapiens)
    - Bile acid and bile salt metabolism (Homo sapiens)
      - Synthesis of bile acids and bile salts (Homo sapiens)
        
        Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol (Homo sapiens)
        
        CYP8B1 12-hydroxylates 4CHOL7aOLONE (Homo sapiens)

General

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Sterol 12alpha hydroxylase (CYP8B1), an enzyme associated with the endoplasmic reticulum membrane, catalyses the 12-alpha-hydroxylation of 7-Alpha-hydroxycholest-4-en-3-one (4CHOL7aOLONE) to 7-alpha,12-alpha-dihydroxycholest-4-en-3-one (4CHOL7a,12aDONE). The human gene has been cloned (Gafvels et al. 1999) and encodes an enzyme with CYP8B1 activity when overexpressed in cultured cells, in agreement with the enzymatic properties of its well-characterised rabbit homolog (Ishida et al. 1992).

Literature References

PubMed ID	Title	Journal	Year
1400444	Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase Coon, MJ, Ishida, H, Okuda, K, Noshiro, M	J. Biol. Chem.	1992
10051404	Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns Gafvels, M, Bjorkhem, I, Jansson, M, Raudsepp, T, Chowdhary, BP, Andersson, U, Persson, B, Eggertsen, G, Olin, M	Genomics	1999