Reactome | ApoB-48 + 40 triacylglycerol + 60 phospholipid => ApoB-48:TG:PL complex

ApoB-48 + 40 triacylglycerol + 60 phospholipid => ApoB-48:TG:PL complex

Stable Identifier

R-HSA-174786

Type

Reaction [transition]

Species

Homo sapiens

Compartment

endoplasmic reticulum lumen, endoplasmic reticulum membrane

ReviewStatus

5/5

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ApoB-48 + 40 triacylglycerol + 60 phospholipid => ApoB-48:TG:PL complex

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Phospholipid (PL) and triacylglycerol (TG) associate with the apo B-48 polypeptide as it is translated. This process is mediated by MTP (microsomal triacylglycerol transfer protein) in the form of a MTP:PDI (protein disulfide isomerase) heterodimer. MTP in vitro binds small amounts of PL and TG (annotated here as as one molecule of each) and efficiently transfers the bound lipid between membranes (Atzel and Wetterau 1994). In vivo, MTP:PDI directly interacts with apoB-48 polypeptide (Wu et al. 1996), and is thought to transfer lipid from the endoplasmic reticulum membrane to nascent apoB-48. While some of the molecular details of MTP function remain unclear, this function is clearly essential in vivo, as patients who lack MTP cannot produce chylomicrons (e.g., Wetterau et al. 1992; Narcisi et al. 1995).

Literature References

PubMed ID	Title	Journal	Year
14732096	Microsomal triglyceride transfer protein: a protein complex required for the assembly of lipoprotein particles Gregg, RE, Gordon, DA, Wetterau, JR	Trends Cell Biol	1995
8626595	Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins Ginsberg, HN, Wu, X, Huang, LS, Wetterau, JR, Zhou, M	J Biol Chem	1996