Reactome | Joining of adjacent Okazaki fragments of the C-strand

Joining of adjacent Okazaki fragments of the C-strand

Stable Identifier

R-HSA-174456

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

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Cell Cycle (Homo sapiens)

- Chromosome Maintenance (Homo sapiens)
  - Telomere Maintenance (Homo sapiens)
    - Extension of Telomeres (Homo sapiens)
      - Telomere C-strand (Lagging Strand) Synthesis (Homo sapiens)
        
        Processive synthesis on the C-strand of the telomere (Homo sapiens)
        
        Joining of adjacent Okazaki fragments of the C-strand (Homo sapiens)

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Joining of adjacent Okazaki fragments of the C-strand

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Removal of the flap by FEN1 leads to the generation of a nick between the 3'-end of the upstream Okazaki fragment and the 5'-end of the downstream Okazaki fragment. DNA ligase I (LIG1) then seals the nicks between adjacent processed Okazaki fragments to generate intact double-stranded DNA (Turchi and Bambara 1993, Bambara et al. 1997, Waga and Stillman 1998, Levin et al. 2000). LIG1 is necessary for ligation of Okazaki fragments at the lagging telomere DNA strand. LIG1 deficiency results in telomere instability, manifested through telomere sister fusions, which is a consequence of DNA breaks in the lagging strand (C-strand) (Le Chalony et al. 2012).

Literature References

PubMed ID	Title	Journal	Year
22460582	Partial complementation of a DNA ligase I deficiency by DNA ligase III and its impact on cell survival and telomere stability in mammalian cells Le Chalony, C, Hoffschir, F, Gauthier, LR, Gross, J, Biard, DS, Boussin, FD, Pennaneach, V	Cell. Mol. Life Sci.	2012