The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer on the G strand of the telomere

Stable Identifier
R-HSA-174427
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
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The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer on the G strand of the telomere
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The complementary C-strand at telomeres is synthesized by the polymerase alpha:primase complex using conventional RNA priming (Nakamura et al. 2005, Dai et al. 2010). This process is regulated by the CST complex (Dai et al. 2010, Feng et al. 2017, Feng et al. 2018) and CDK1 (Dai et al. 2010, Dai et al. 2012).

Literature References
PubMed ID Title Journal Year
22871736 CDK1 differentially regulates G-overhang generation at leading- and lagging-strand telomeres in telomerase-negative cells in G2 phase

Dai, X, Huang, C, Chai, W

Cell Cycle 2012
16314528 Alterations of DNA and chromatin structures at telomeres and genetic instability in mouse cells defective in DNA polymerase alpha

Nakamura, M, Nabetani, A, Mizuno, T, Hanaoka, F, Ishikawa, F

Mol. Cell. Biol. 2005
28334750 CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance

Feng, X, Hsu, SJ, Kasbek, C, Chaiken, M, Price, CM

Nucleic Acids Res. 2017
30026550 CTC1-STN1 terminates telomerase while STN1-TEN1 enables C-strand synthesis during telomere replication in colon cancer cells

Feng, X, Hsu, SJ, Bhattacharjee, A, Wang, Y, Diao, J, Price, CM

Nat Commun 2018
20639858 Molecular steps of G-overhang generation at human telomeres and its function in chromosome end protection

Dai, X, Huang, C, Bhusari, A, Sampathi, S, Schubert, K, Chai, W

EMBO J. 2010
Participants
Input
Output
Participant Of
hasEvent
Event Information
Go Biological Process
telomere maintenance via semi-conservative replication (0032201)
Catalyst Activity
Catalyst Activity
Title
DNA-directed DNA polymerase activity of RNA primer:G-strand extended telomere end:POLA:primase [nucleoplasm]
Physical Entity
RNA primer:G-strand extended telomere end:POLA:primase [nucleoplasm]
Activity
DNA-directed DNA polymerase activity (0003887)
Orthologous Events
The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer on the G strand of the telomere (Canis familiaris)
The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer on the G strand of the telomere (Danio rerio)
The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer on the G strand of the telomere (Mus musculus)
The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer on the G strand of the telomere (Rattus norvegicus)
The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer on the G strand of the telomere (Sus scrofa)
The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer on the G strand of the telomere (Xenopus tropicalis)
Authored
Blackburn, EH  (2006-03-10)
Seidel, J  (2006-03-10)
Reviewed
Price, C  (2006-07-13)
Hayashi, MT  (2020-04-29)
Created
Gillespie, ME  (2006-02-17)
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