Reactome | Assembly of an Active Transcription Complex

Assembly of an Active Transcription Complex

Stable Identifier

R-HSA-168326

Type

Reaction [binding]

Species

Homo sapiens

Related Species

Influenza A virus

Compartment

nucleoplasm

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Assembly of an Active Transcription Complex

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The 5' end of the vRNA associates with a binding site on the PB1 subunit of the viral RNA polymerase, distinct from the 3' vRNA binding site, which is subsequenty bound forming a loop. These binding events set off allosteric conformational changes in the trimeric polymerase complex that induce PB2 binding of the methylated cap on a host pre-mRNA (Plotch, 1981; Cianci, 1995; Li, 1998; Brownlee, 2002; Kolpashchikov, 2004). PB2 amino acids 242-282 and 538-577 are involved in cap binding (Honda, 1999). Direct or indirect interaction with active, transcribing host RNA polymerase II is thought to supply host mRNA for the caps (Bouloy, 1978; Engelhardt, 2005).

Literature References

PubMed ID	Title	Journal	Year
10526235	Two separate sequences of PB2 subunit constitute the RNA cap-binding site of influenza virus RNA polymerase Honda, A, Mizumoto, K, Ishihama, A	Genes Cells	1999
7769657	Differential activation of the influenza virus polymerase via template RNA binding Cianci, C, Tiley, L, Krystal, M	J Virol	1995
12072510	The RNA polymerase of influenza a virus is stabilized by interaction with its viral RNA promoter Brownlee, GG, Sharps, JL	J Virol	2002
9755184	RNA-dependent activation of primer RNA production by influenza virus polymerase: different regions of the same protein subunit constitute the two required RNA-binding sites Li, ML, Ramirez, BC, Krug, RM	EMBO J	1998
6261960	A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription Plotch, SJ, Bouloy, M, Ulmanen, I, Krug, RM	Cell	1981

Participants

Input

Output

vRNA Transcription Complex [nucleoplasm] (Influenza A virus)

Participates

as an event of

Viral Messenger RNA Synthesis (Homo sapiens)

Event Information

Go Biological Process

viral transcription (0019083)

This event is regulated

Positively by

Regulator

NP [nucleoplasm] (Influenza A virus)

Regulator

RNA Polymerase II (phosphorylated):TFIIF:capped pre-mRNA [nucleoplasm] (Homo sapiens)

Disease

Name	Identifier	Synonyms
influenza	DOID:8469	Influenza with other manifestations (disorder), Influenza with other manifestations NOS (disorder), flu, Influenza with other manifestations, Influenza with non-respiratory manifestation (disorder), influenza with non-respiratory manifestation

Authored

Bortz, E (2007-02-13)

Garcia-Sastre, A (2007-02-13)

Reviewed

Squires, B (2007-02-13)

Created