At the plasma membrane, phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase aka phosphatase and tensin homolog (PTEN) dephosphorylates phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) to phosphatidylinositol 4-phosphate (PI4P) (Myers et al. 1998, Das et al. 2003). Early studies indicated that magnesium ion, Mg2+, was needed for the catalytic activity of PTEN isolated from bovine thymus (Kabuyama et al. 1996). Subsequent studies have shown that PTEN was catalytically active in buffers free of magnesium and magnesium was not detected as part of the PTEN crystal (Lee et al. 1999).
Fukui, Y, Kabuyama, Y, Homma, Y, Nakatsu, N
Stolarov, JP, van der Kaay, J, Myers, MP, Pass, I, Downes, CP, Batty, IH, Wigler, MH, Tonks, NK, Hemmings, BA
Das, S, Cho, W, Dixon, JE
Georgescu, MM, Pavletich, NP, Shi, Y, Maehama, T, Pandolfi, P, Yang, H, Lee, JO, Dixon, JE, Di Cristofano, A
phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity of PTEN [cytosol]
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