Reactome | Addition of nucleotides 5 through 9 on the growing HIV-1 transcript

Addition of nucleotides 5 through 9 on the growing HIV-1 transcript

Stable Identifier

R-HSA-167136

Type

Reaction [transition]

Species

Homo sapiens

Related Species

Human immunodeficiency virus 1

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

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Disease (Homo sapiens)

- Infectious disease (Homo sapiens)
  - Viral Infection Pathways (Homo sapiens)
    - HIV Infection (Homo sapiens)
      - HIV Life Cycle (Homo sapiens)
        
        Late Phase of HIV Life Cycle (Homo sapiens)
        
        Transcription of the HIV genome (Homo sapiens)
        
        RNA Polymerase II HIV Promoter Escape (Homo sapiens)
        
        Addition of nucleotides 5 through 9 on the growing HIV-1 transcript (Homo sapiens)

General

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Addition of nucleotides 5 through 9 on the growing HIV-1 transcript

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Formation of the second phosphodiester bond creates a 3-nt product. This transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. At this stage pausing by RNA polymerase II may result in repeated slippage and reextension of the nascent RNA. The transcription complex still requires continued ATP-hydrolysis by TFIIH for efficient promoter escape. Basal transcription factor TFIIE dissociates from the initiation complex before position +10.

Basal transcription factor TFIIF may reassociate and can stimulate transcription elongation at multiple stages. The open region (“transcription bubble”) expands concomitant with the site of RNA-extension, eventually reaching an open region from -9 to +9.

Literature References

PubMed ID	Title	Journal	Year
9256425	A role for TFIIH in controlling the activity of early RNA polymerase II elongation complexes. Conaway, JW, Dvir, A, Conaway, RC	Proc Natl Acad Sci U S A	1997
11739720	Strong natural pausing by RNA polymerase II within 10 bases of transcription start may result in repeated slippage and reextension of the nascent RNA. Pal, M, Luse, DS	Mol Cell Biol	2001
7601352	Recycling of the general transcription factors during RNA polymerase II transcription. Zawel, L, Kumar, KP, Reinberg, D	Genes Dev	1995
9405375	Three transitions in the RNA polymerase II transcription complex during initiation. Fiedler, U, Timmers, HT, Holstege, FC	EMBO J	1998

Participants

Input

Output

Participates

as an event of

RNA Polymerase II HIV Promoter Escape (Homo sapiens)

Catalyst Activity

DNA-directed 5'-3' RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

Physical Entity

RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] (Homo sapiens)

Activity

DNA-directed 5'-3' RNA polymerase activity (GO:0003899)

Inferred From

Addition of Nucleotides 5 through 9 on the growing Transcript (Homo sapiens)

Disease

Name	Identifier	Synonyms
Human immunodeficiency virus infectious disease	DOID:526	HIV infection

Authored

Matthews, L (2005-07-27)

Rice, AP (2005-07-27)

Created

Matthews, L (2005-10-11)