Reactome | Addition of the third nucleotide on the nascent HIV-1 transcript

Addition of the third nucleotide on the nascent HIV-1 transcript

Stable Identifier

R-HSA-167121

Type

Reaction [omitted]

Species

Homo sapiens

Related Species

Human immunodeficiency virus 1

Compartment

nucleoplasm

Locations in the PathwayBrowser

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Disease (Homo sapiens)

- Infectious disease (Homo sapiens)
  - HIV Infection (Homo sapiens)
    - HIV Life Cycle (Homo sapiens)
      - Late Phase of HIV Life Cycle (Homo sapiens)
        
        Transcription of the HIV genome (Homo sapiens)
        
        RNA Polymerase II HIV Promoter Escape (Homo sapiens)
        
        Addition of the third nucleotide on the nascent HIV-1 transcript (Homo sapiens)

General

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Addition of the third nucleotide on the nascent HIV-1 transcript

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The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Formation of the second phosphodiester bond creates a 3-nt product. This short transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. The transcription complex still requires continued ATP-hydrolysis by TFIIH and remains sensitive to single-stranded oligo-nucleotide inhibition.

The open region (“transcription bubble”) expands concomitant with the site of RNA-extension. In this case this region spans positions -9 to +3.

Literature References

PubMed ID	Title	Journal	Year
9405375	Three transitions in the RNA polymerase II transcription complex during initiation. Holstege, FC, Fiedler, U, Timmers, HT	EMBO J	1998
11784853	Translocation after synthesis of a four-nucleotide RNA commits RNA polymerase II to promoter escape. Kugel, JF, Goodrich, JA	Mol Cell Biol	2002

Participants

Input

Output

Participant Of

hasEvent

RNA Polymerase II HIV Promoter Escape

Catalyst Activity

Title

DNA-directed 5'-3' RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

Physical Entity

RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

Activity

DNA-directed 5'-3' RNA polymerase activity (0003899)

Inferred From

Addition of the third nucleotide on the nascent transcript (Homo sapiens)

Disease

Name	Identifier	Synonyms
Human immunodeficiency virus infectious disease	526	HIV infection

Authored

Matthews, L (2005-07-27)

Rice, AP (2005-07-27)

Created

Matthews, L (2005-10-11)