Reactome | Addition of the third nucleotide on the nascent HIV-1 transcript

Addition of the third nucleotide on the nascent HIV-1 transcript

Stable Identifier

R-HSA-167121

Type

Reaction [omitted]

Species

Homo sapiens

Related Species

Human immunodeficiency virus 1

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

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Disease (Homo sapiens)

- Infectious disease (Homo sapiens)
  - Viral Infection Pathways (Homo sapiens)
    - HIV Infection (Homo sapiens)
      - HIV Life Cycle (Homo sapiens)
        
        Late Phase of HIV Life Cycle (Homo sapiens)
        
        Transcription of the HIV genome (Homo sapiens)
        
        RNA Polymerase II HIV Promoter Escape (Homo sapiens)
        
        Addition of the third nucleotide on the nascent HIV-1 transcript (Homo sapiens)

General

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Addition of the third nucleotide on the nascent HIV-1 transcript

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Formation of the second phosphodiester bond creates a 3-nt product. This short transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. The transcription complex still requires continued ATP-hydrolysis by TFIIH and remains sensitive to single-stranded oligo-nucleotide inhibition.

The open region (“transcription bubble”) expands concomitant with the site of RNA-extension. In this case this region spans positions -9 to +3.

Literature References

PubMed ID	Title	Journal	Year
11784853	Translocation after synthesis of a four-nucleotide RNA commits RNA polymerase II to promoter escape. Goodrich, JA, Kugel, JF	Mol Cell Biol	2002
9405375	Three transitions in the RNA polymerase II transcription complex during initiation. Fiedler, U, Timmers, HT, Holstege, FC	EMBO J	1998