Annealing of 3'-end of unwound transfer RNA primer with genomic RNA

Stable Identifier
R-HSA-164527
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
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Retroviruses use cellular tRNAs as primers for reverse transcription of the viral genomic RNA (Mak and Kleiman 1997). The primer tRNA is selectively packaged during assembly of retrovirus particles. In the case of HIV-1, lysine tRNAs are preferentially incorporated during retroviral packaging, and lysine tRNA 3, the specific isoacceptor form that serves as a primer for reverse transcription, anneals to the PBS (primer binding site) within the U5 region of the viral genomic RNA. This association appears to be mediated by the viral reverse transcriptase (RT) protein, possibly its "thumb" and "connection" domains (Jiang et al. 1993; Mak et al. 1994; Mishima and Steitz 1995).

Literature References
PubMed ID Title Journal Year
7511167 Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles

Mak, J, Jiang, M, Wainberg, MA, Hammarskjold, ML, Rekosh, D, Kleiman, L

J Virol 1994
8497049 Identification of tRNAs incorporated into wild-type and mutant human immunodeficiency virus type 1

Jiang, M, Mak, J, Ladha, A, Cohen, E, Klein, M, Rovinski, B, Kleiman, L

J Virol 1993
Participants
Participant Of
Event Information
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease 526 HIV infection
Authored
Reviewed
Created