Annealing of 3'-end of unwound transfer RNA primer with genomic RNA

Stable Identifier
R-HSA-164527
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
cytosol
ReviewStatus
5/5
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Annealing of 3'-end of unwound transfer RNA primer with genomic RNA
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
Retroviruses use cellular tRNAs as primers for reverse transcription of the viral genomic RNA (Mak and Kleiman 1997). The primer tRNA is selectively packaged during assembly of retrovirus particles. In the case of HIV-1, lysine tRNAs are preferentially incorporated during retroviral packaging, and lysine tRNA 3, the specific isoacceptor form that serves as a primer for reverse transcription, anneals to the PBS (primer binding site) within the U5 region of the viral genomic RNA. This association appears to be mediated by the viral reverse transcriptase (RT) protein, possibly its "thumb" and "connection" domains (Jiang et al. 1993; Mak et al. 1994; Mishima and Steitz 1995).
Literature References
PubMed ID Title Journal Year
8497049 Identification of tRNAs incorporated into wild-type and mutant human immunodeficiency virus type 1

Jiang, M, Mak, J, Ladha, A, Cohen, E, Klein, M, Rovinski, B, Kleiman, L

J Virol 1993
7511167 Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles

Mak, J, Jiang, M, Wainberg, MA, Hammarskjold, ML, Rekosh, D, Kleiman, L

J Virol 1994
Participants
Input
Output
Participates
as an event of
Event Information
Go Biological Process
RNA-templated DNA biosynthetic process (0006278)
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease DOID:526 HIV infection
Cross References
Mondo
Authored
Gopinathrao, G  (2006-05-19)
D'Eustachio, P  (2006-05-19)
Reviewed
Hughes, SH  (2006-10-31)
Created
D'Eustachio, P  (2005-05-24)
Cite Us!