3' PPT-primed initiation of plus-strand DNA synthesis

Stable Identifier
R-HSA-164513
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
cytosol
Synonyms
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3' PPT-primed initiation of plus-strand DNA synthesis
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HIV-1 genomic RNA contains a centrally located PPT (cPPT) within the pol gene that, like 3'PPT, is spared by RNase H during minus-strand DNA synthesis and persists to prime plus-strand DNA synthesis. This ribonucleotide primes the synthesis of a plus-strand DNA extending through the U3 and R regions of the HIV sequence and terminating in the PBS region (the tRNA primer-binding site). This DNA segment is known as plus-strand strong-stop DNA (+sssDNA) (Telesnitsky and Goff 1997; Pullen et al. 1993; Huber and Richardson 1990). cPPT priming is important for efficient viral replication (Alizon et al. 1992; Rausch and Le Grice 2004). Several features of cPPT priming in vivo remain to be clarified.

Literature References
PubMed ID Title Journal Year
7681062 The sequence features important for plus strand priming by human immunodeficiency virus type 1 reverse transcriptase

Pullen, KA, Rattray, AJ, Champoux, JJ

J Biol Chem 1993
10723025 The HIV-1 reverse transcription (RT) process as target for RT inhibitors

Jonckheere, H, Anne, J, De Clercq, E

Med Res Rev 2000
1693920 Processing of the primer for plus strand DNA synthesis by human immunodeficiency virus 1 reverse transcriptase

Huber, HE, Richardson, CC

J Biol Chem 1990
  Retroviruses

Coffin, JM, Hughes, SH, Varmus, HE

  1997
Participants
Input
Output
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
DNA-directed DNA polymerase activity of RTC with extending minus strand DNA [cytosol]
Physical Entity
RTC with extending minus strand DNA [cytosol]
Activity
DNA-directed DNA polymerase activity (0003887)
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease 526 HIV infection
Authored
Gopinathrao, G  (2006-05-19)
D'Eustachio, P  (2006-05-19)
Reviewed
Hughes, SH  (2006-10-31)
Created
D'Eustachio, P  (2005-05-24)
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