3' PPT-primed initiation of plus-strand DNA synthesis

Stable Identifier
Reaction [transition]
Homo sapiens
Related Species
Human immunodeficiency virus 1
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
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HIV-1 genomic RNA contains a centrally located PPT (cPPT) within the pol gene that, like 3'PPT, is spared by RNase H during minus-strand DNA synthesis and persists to prime plus-strand DNA synthesis. This ribonucleotide primes the synthesis of a plus-strand DNA extending through the U3 and R regions of the HIV sequence and terminating in the PBS region (the tRNA primer-binding site). This DNA segment is known as plus-strand strong-stop DNA (+sssDNA) (Telesnitsky and Goff 1997; Pullen et al. 1993; Huber and Richardson 1990). cPPT priming is important for efficient viral replication (Alizon et al. 1992; Rausch and Le Grice 2004). Several features of cPPT priming in vivo remain to be clarified.

Literature References
PubMed ID Title Journal Year
10723025 The HIV-1 reverse transcription (RT) process as target for RT inhibitors

De Clercq, E, Anne, J, Jonckheere, H

Med Res Rev 2000
7681062 The sequence features important for plus strand priming by human immunodeficiency virus type 1 reverse transcriptase

Pullen, KA, Champoux, JJ, Rattray, AJ

J Biol Chem 1993
1693920 Processing of the primer for plus strand DNA synthesis by human immunodeficiency virus 1 reverse transcriptase

Huber, HE, Richardson, CC

J Biol Chem 1990

Varmus, HE, Hughes, SH, Coffin, JM

Catalyst Activity

DNA-directed DNA polymerase activity of RTC with extending minus strand DNA [cytosol]

Name Identifier Synonyms
Human immunodeficiency virus infectious disease DOID:526 HIV infection
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