Reactome | PP2A-B56 dephosphorylates centromeric cohesin

PP2A-B56 dephosphorylates centromeric cohesin

Stable Identifier

R-HSA-1638821

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, chromosome

ReviewStatus

5/5

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PP2A-B56 dephosphorylates centromeric cohesin

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PLK1-mediated phosphorylation of the STAG2 subunit of centromeric cohesin (Hauf et al. 2005) is counteracted by the kinetochore PP2A phosphatase, containing the 56 kDa regulatory B subunit (PP2A-B56 i.e. PP2A-PPP2R5). PP2A-B56 is recruited to the centromeric cohesin complex by shugoshin proteins (SGOL1 and SGOL2) (Kitajima et al. 2006), which are also kinetochore constituents (Cheeseman and Desai 2008). SGOL1 localization to centromeres is sustained by the interaction with histone H2A possessing the phosphorylation of T120 which is introduced by the protein kinase BUB1, and heterochromatin protein HP1 (Kitajima et al. 2005, Kawashima et al. 2010, Yamagishi et al. 2008). Shugoshin- and PP2A-B56-regulated dephosphorylation of centromeric STAG2 ensures that the cohesin complex remains bound to centromeres throughout prometaphase and metaphase, thereby preventing premature separation of sister chromatids (Salic et al. 2004, Kitajima et al. 2004, Kitajima et al. 2005, Kitajima et al. 2006).

Literature References

PubMed ID	Title	Journal	Year
15723797	Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization Kitajima, TS, Hauf, S, Ohsugi, M, Yamamoto, T, Watanabe, Y	Curr. Biol.	2005
19965387	Phosphorylation of H2A by Bub1 prevents chromosomal instability through localizing shugoshin Kawashima, SA, Yamagishi, Y, Honda, T, Ishiguro, K, Watanabe, Y	Science	2010
16541025	Shugoshin collaborates with protein phosphatase 2A to protect cohesin Kitajima, TS, Sakuno, T, Ishiguro, K, Iemura, S, Natsume, T, Kawashima, SA, Watanabe, Y	Nature	2006
15339662	Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis Salic, A, Waters, JC, Mitchison, TJ	Cell	2004
14730319	The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis Kitajima, TS, Kawashima, SA, Watanabe, Y	Nature	2004
18097444	Molecular architecture of the kinetochore-microtubule interface Cheeseman, IM, Desai, A	Nat Rev Mol Cell Biol	2008
15737063	Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2 Hauf, S, Roitinger, E, Koch, B, Dittrich, CM, Mechtler, K, Peters, JM	PLoS Biol	2005
18716626	Heterochromatin links to centromeric protection by recruiting shugoshin Yamagishi, Y, Sakuno, T, Shimura, M, Watanabe, Y	Nature	2008

Participants

Input

Output

Participates

as an event of

Resolution of Sister Chromatid Cohesion (Homo sapiens)

Catalyst Activity

protein serine/threonine phosphatase activity of p-STAG2,RAD21-Ac-Cohesin:PDS5:p-CDCA5:WAPAL:Sister Centromeres:Kinetochores:Microtubules [chromosome, centromeric region]

Physical Entity

p-STAG2,RAD21-Ac-Cohesin:PDS5:p-CDCA5:WAPAL:Sister Centromeres:Kinetochores:Microtubules [chromosome, centromeric region] (Homo sapiens)

Activity

protein serine/threonine phosphatase activity (GO:0004722)

Orthologous Events

PP2A-B56 dephosphorylates centromeric cohesin (Bos taurus)

PP2A-B56 dephosphorylates centromeric cohesin (Canis familiaris)

PP2A-B56 dephosphorylates centromeric cohesin (Danio rerio)

PP2A-B56 dephosphorylates centromeric cohesin (Gallus gallus)

PP2A-B56 dephosphorylates centromeric cohesin (Mus musculus)

PP2A-B56 dephosphorylates centromeric cohesin (Rattus norvegicus)

PP2A-B56 dephosphorylates centromeric cohesin (Sus scrofa)