phosphatidylinositol + UDP-N-acetyl-D-glucosamine -> N-acetylglucosaminyl-PI + UDP

Stable Identifier
R-HSA-162730
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The first step of GPI synthesis is the transfer of N-acetylglucosamine from cytosolic UDP-N-acetylglucosamine to phosphatidyl inositol (PI) in the endoplasmic reticulum membrane. The reaction is catalyzed by a multimeric enzyme, also localized to the endoplasmic reticulum membrane, seven components of which have been identified to date by mutagenesis studies in cultured cells and by co-precipitation and reconstitution studies in vitro (Murakami et al. 2005; Watanabe et al. 1996, 1998, 2000).

Literature References
PubMed ID Title Journal Year
8900170 PIG-A and PIG-H, which participate in glycosylphosphatidylinositol anchor biosynthesis, form a protein complex in the endoplasmic reticulum

Watanabe, R, Kinoshita, T, Masaki, R, Yamamoto, A, Takeda, J, Inoue, N

J Biol Chem 1996
9463366 The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1

Watanabe, R, Inoue, N, Westfall, B, Taron, CH, Orlean, P, Takeda, J, Kinoshita, T

EMBO J 1998
16162815 The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component

Murakami, Y, Siripanyaphinyo, U, Hong, Y, Tashima, Y, Maeda, Y, Kinoshita, T

Mol. Biol. Cell 2005
10944123 Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2

Watanabe, R, Murakami, Y, Marmor, MD, Inoue, N, Maeda, Y, Hino, J, Kangawa, K, Julius, M, Kinoshita, T

EMBO J 2000
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
UDP-glycosyltransferase activity of GPI-GnT [endoplasmic reticulum membrane]
Physical Entity
Activity
Orthologous Events
Authored
Created
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