phosphatidylinositol + UDP-N-acetyl-D-glucosamine -> N-acetylglucosaminyl-PI + UDP

Stable Identifier
Reaction [transition]
Homo sapiens
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The first step of GPI synthesis is the transfer of N-acetylglucosamine from cytosolic UDP-N-acetylglucosamine to phosphatidyl inositol (PI) in the endoplasmic reticulum membrane. The reaction is catalyzed by a multimeric enzyme, also localized to the endoplasmic reticulum membrane, seven components of which have been identified to date by mutagenesis studies in cultured cells and by co-precipitation and reconstitution studies in vitro (Murakami et al. 2005; Watanabe et al. 1996, 1998, 2000).
Literature References
PubMed ID Title Journal Year
9463366 The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1

Kinoshita, T, Takeda, J, Taron, CH, Orlean, P, Westfall, B, Inoue, N, Watanabe, R

EMBO J 1998
8900170 PIG-A and PIG-H, which participate in glycosylphosphatidylinositol anchor biosynthesis, form a protein complex in the endoplasmic reticulum

Kinoshita, T, Takeda, J, Inoue, N, Watanabe, R, Yamamoto, A, Masaki, R

J Biol Chem 1996
16162815 The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component

Kinoshita, T, Murakami, Y, Tashima, Y, Maeda, Y, Siripanyaphinyo, U, Hong, Y

Mol. Biol. Cell 2005
10944123 Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2

Kinoshita, T, Murakami, Y, Kangawa, K, Hino, J, Inoue, N, Watanabe, R, Marmor, MD, Maeda, Y, Julius, M

EMBO J 2000
Catalyst Activity

UDP-glycosyltransferase activity of GPI-GnT [endoplasmic reticulum membrane]

Orthologous Events
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