Reactome | Synthesis of dolichyl-phosphate mannose

Synthesis of dolichyl-phosphate mannose

Stable Identifier

R-HSA-162699

Type

Pathway

Species

Homo sapiens

Locations in the PathwayBrowser

Expand all

Metabolism of proteins (Homo sapiens)

- Post-translational protein modification (Homo sapiens)
  - Asparagine N-linked glycosylation (Homo sapiens)
    - Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein (Homo sapiens)
      - Synthesis of substrates in N-glycan biosythesis (Homo sapiens)
        
        Synthesis of dolichyl-phosphate mannose (Homo sapiens)
  - Post-translational modification: synthesis of GPI-anchored proteins (Homo sapiens)
    - Synthesis of dolichyl-phosphate mannose (Homo sapiens)

General

SBML | | PDF

SVG | | PPTX | SBGN

Click the image above or here to open this pathway in the Pathway Browser

Dolichyl-phosphate-mannose (DPM, DOLPman) is the donor of mannose groups in the synthesis of the dolichyl pyrophosphate-linked precursor oligosaccharide in asparagine-linked glycosylation, in the synthesis of the glycosyl phosphatidylinositol (GPI) anchor precursor, in protein O-mannosylation and in protein C-mannosylation. Its synthesis proceeds in two steps. First, cytosolic GDP-mannose reacts with dolichyl phosphate exposed on the cytosolic face of the endoplasmic reticulum membrane to form DPM with its mannose moiety oriented toward the cytosol. The DPM molecule then flips in the endoplasmic reticulum membrane, so that its mannose moiety is in the endoplasmic reticulum lumen, accessible to the enzymes that catalyze its transfer to growing glycolipids and glycoproteins (Kinoshita and Inoue, 2000; Maeda et al, 2000).

Literature References

PubMed ID	Title	Journal	Year
11102867	Dissecting and manipulating the pathway for glycosylphosphatidylinositol-anchor biosynthesis Kinoshita, T, Inoue, N	Curr Opin Chem Biol	2000
10835346	Human dolichol-phosphate-mannose synthase consists of three subunits, DPM1, DPM2 and DPM3 Maeda, Y, Tanaka, S, Hino, J, Kangawa, K, Kinoshita, T	EMBO J	2000