NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B)

Stable Identifier
Reaction [transition]
Homo sapiens
Recruitment of TAP to the EJC
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The mRNA is transferred from ALYREF of the TREX complex to NXF1 (TAP) of the NXF1:NXT1 export complex (Hautbergue et al. 2008). Interaction between the TREX complex and NXF1 exposes the arginine-rich RNA-binding domain of NXF1 (Viphakone et al. 2012). Methylation of arginine residues on ALYREF also appears to be necessary for dissociation of mRNA from ALYREF during the transfer (Hung et al. 2010). The interaction between ALYREF and NXF1 occurs in the vicinity of nuclear speckles (Teng and Wilson 2013). DDX39B (UAP56) binds (Kota et al. 2008) and hydrolyzes ATP (Taniguchi and Ohno 2008). UAP56 is believed to hydrolyze ATP and UAP56:ADP is believed to dissociate at some point during the transfer of the mRNA to NXF1 (Taniguchi and Ohno 2008, Chang et al. 2013).

Literature References
PubMed ID Title Journal Year
10323864 The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences

Cullen, BR, Kang, Y

Genes Dev. 1999
9660949 TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus

Izaurralde, E, Saavedra, C, Schmitt, C, GrĂ¼ter, P, Wilm, M, Tabernero, C, Bachi, A, Felber, BK, von Kobbe, C

Mol. Cell 1998
10489353 Analysis of the RNA binding specificity of the human tap protein, a constitutive transport element-specific nuclear RNA export factor

Cullen, BR, Yang, J, Bogerd, HP, Kang, Y

Virology 1999
23826332 Mapping interactions between mRNA export factors in living cells

Wilson, SA, Teng, IF

PLoS ONE 2013
11073998 TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture

Braun, IC, Herold, A, Carmo-Fonseca, M, Izaurralde, E, Kutay, U, Suyama, M, Rodrigues, JP, Bork, P

Mol. Cell. Biol. 2000
10228171 The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human

Strässer, K, Podtelejnikov, A, Jung, JU, Katahira, J, Hurt, E, Mann, M

EMBO J. 1999
23299939 Chtop is a component of the dynamic TREX mRNA export complex

Chang, CT, Wilson, SA, Walsh, MJ, van Dijk, TB, Philipsen, S, Viphakone, N, Hautbergue, GM

EMBO J. 2013
22893130 TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export

Reed, R, Folco, EG, Holland, A, Chang, CT, Wilson, SA, Walsh, M, Hautbergue, GM, Viphakone, N

Nat Commun 2012
17000901 The solution structure of REF2-I reveals interdomain interactions and regions involved in binding mRNA export factors and RNA

Golovanov, AP, Lian, LY, Tintaru, AM, Wilson, SA, Hautbergue, GM

RNA 2006
18364396 Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP

Golovanov, AP, Lian, LY, Wilson, SA, Hung, ML, Hautbergue, GM

Proc. Natl. Acad. Sci. U.S.A. 2008
18411249 Binding of ATP to UAP56 is necessary for mRNA export

Nickerson, JA, Wagner, SR, Underwood, JM, Kota, KP, Huerta, E

J. Cell. Sci. 2008
11739738 Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes

Coburn, GA, Wiegand, HL, Bogerd, HP, Zeng, Y, Kang, Y, Cullen, BR

Mol. Cell. Biol. 2002
20129943 Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1

Snijders, AP, Dickman, MJ, Wilson, SA, Hung, ML, Hautbergue, GM

Nucleic Acids Res. 2010
Event Information
Go Biological Process
Catalyst Activity

ATP hydrolysis activity of polyadenylated, capped mRNA:CBC:EJC:TREX:SRSF proteins [nucleoplasm]

Orthologous Events
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