Reactome | NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B)

NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B)

Stable Identifier

R-HSA-159101

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

Synonyms

Recruitment of TAP to the EJC

ReviewStatus

5/5

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NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B)

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The mRNA is transferred from ALYREF of the TREX complex to NXF1 (TAP) of the NXF1:NXT1 export complex (Hautbergue et al. 2008). Interaction between the TREX complex and NXF1 exposes the arginine-rich RNA-binding domain of NXF1 (Viphakone et al. 2012). Methylation of arginine residues on ALYREF also appears to be necessary for dissociation of mRNA from ALYREF during the transfer (Hung et al. 2010). The interaction between ALYREF and NXF1 occurs in the vicinity of nuclear speckles (Teng and Wilson 2013). DDX39B (UAP56) binds (Kota et al. 2008) and hydrolyzes ATP (Taniguchi and Ohno 2008). UAP56 is believed to hydrolyze ATP and UAP56:ADP is believed to dissociate at some point during the transfer of the mRNA to NXF1 (Taniguchi and Ohno 2008, Chang et al. 2013).

Literature References

PubMed ID	Title	Journal	Year
10323864	The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences Kang, Y, Cullen, BR	Genes Dev.	1999
9660949	TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus Grüter, P, Tabernero, C, von Kobbe, C, Schmitt, C, Saavedra, C, Bachi, A, Wilm, M, Felber, BK, Izaurralde, E	Mol. Cell	1998
10489353	Analysis of the RNA binding specificity of the human tap protein, a constitutive transport element-specific nuclear RNA export factor Kang, Y, Bogerd, HP, Yang, J, Cullen, BR	Virology	1999
23826332	Mapping interactions between mRNA export factors in living cells Teng, IF, Wilson, SA	PLoS ONE	2013
11073998	TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture Herold, A, Suyama, M, Rodrigues, JP, Braun, IC, Kutay, U, Carmo-Fonseca, M, Bork, P, Izaurralde, E	Mol. Cell. Biol.	2000
10228171	The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human Katahira, J, Strässer, K, Podtelejnikov, A, Mann, M, Jung, JU, Hurt, E	EMBO J.	1999
23299939	Chtop is a component of the dynamic TREX mRNA export complex Chang, CT, Hautbergue, GM, Walsh, MJ, Viphakone, N, van Dijk, TB, Philipsen, S, Wilson, SA	EMBO J.	2013
22893130	TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export Viphakone, N, Hautbergue, GM, Walsh, M, Chang, CT, Holland, A, Folco, EG, Reed, R, Wilson, SA	Nat Commun	2012
17000901	The solution structure of REF2-I reveals interdomain interactions and regions involved in binding mRNA export factors and RNA Golovanov, AP, Hautbergue, GM, Tintaru, AM, Lian, LY, Wilson, SA	RNA	2006
18364396	Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP Hautbergue, GM, Hung, ML, Golovanov, AP, Lian, LY, Wilson, SA	Proc. Natl. Acad. Sci. U.S.A.	2008
18411249	Binding of ATP to UAP56 is necessary for mRNA export Kota, KP, Wagner, SR, Huerta, E, Underwood, JM, Nickerson, JA	J. Cell. Sci.	2008
11739738	Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes Wiegand, HL, Coburn, GA, Zeng, Y, Kang, Y, Bogerd, HP, Cullen, BR	Mol. Cell. Biol.	2002
20129943	Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1 Hung, ML, Hautbergue, GM, Snijders, AP, Dickman, MJ, Wilson, SA	Nucleic Acids Res.	2010