Reactome | CA1:Zn2+,CA2:Zn2+ dehydrate HCO3- to CO2

CA1:Zn2+,CA2:Zn2+ dehydrate HCO3- to CO2

Stable Identifier

R-HSA-1475436

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

Carbonic anhydrase I/II dehydrates bicarbonate

ReviewStatus

5/5

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CA1:Zn2+,CA2:Zn2+ dehydrate HCO3- to CO2

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Carbonic anhydrase I (CA1, Khalifah 1971, Pesando 1975, Simonsson et al. 1982, Ren & Lindskog 1992) and carbonic anhydrase II (CA2, Tibell et al. 1984, Jones & Shaw 1983, Ghannam et al. 1986) hydrate carbon dioxide (CO2) to yield bicarbonate (HCO3-) and a proton (H+). During the reaction a hydroxyl group bound by the zinc ion (Zn2+) attacks the CO2 molecule in the active site to directly form HCO3- (reviewed in Lindskog 1997). The HCO3- is displaced by water, which is then deprotonated by a histidine residue to recreate the Zn2+:hydroxyl group. Depending on the concentrations of reactants the reaction is reversible.

Literature References

PubMed ID	Title	Journal	Year
6819139	A 13C nuclear magnetic resonance study of CO2/HCO-3 exchange catalyzed by human carbonic anhydrase I Lindskog, S, Simonsson, I, Jonsson, BH	Eur J Biochem	1982
234739	Proton magnetic resonance studies of carbonic anhydrase. II. Group controlling catalytic activity Pesando, JM	Biochemistry	1975
6407977	A chemical and enzymological comparison of the common major human erythrocyte carbonic anhydrase II, its minor component, and a new genetic variant, CA II Melbourne (237 Pro leads to His) Jones, GL, Shaw, DC	Hum Genet	1983
1554744	Buffer dependence of CO2 hydration catalyzed by human carbonic anhydrase I Lindskog, S, Ren, X	Biochim Biophys Acta	1992
6433979	Anion inhibition of CO2 hydration catalyzed by human carbonic anhydrase II. Mechanistic implications Forsman, C, Lindskog, S, Simonsson, I, Tibell, L	Biochim Biophys Acta	1984
4994926	The carbon dioxide hydration activity of carbonic anhydrase. I. Stop-flow kinetic studies on the native human isoenzymes B and C Khalifah, RG	J Biol Chem	1971
3080418	Activation parameters for the carbonic anhydrase II-catalyzed hydration of CO2 Rowlett, RS, Tsen, W, Ghannam, AF	J Biol Chem	1986
9336012	Structure and mechanism of carbonic anhydrase Lindskog, S	Pharmacol Ther	1997