ERBB2 homodimers trans-autophosphorylate to form phosphorylated ERBB2 that activates downstream signaling cascades (Hazan et al. 1990, Ricci et al. 1995, Pickl and Ries 2009, Maadi et al. 2018). The best characterized trans-autophophosphorylation sites in ERBB2 heterodimers are tyrosines Y1023, Y1139, Y1196, Y1221, Y1222 and Y1248. Studies of ERBB2 homodimers have not investigated trans-autophosphorylated tyrosines of ERBB2 comprehensively. Instead, each study reported a subset of trans-autophosphorylation sites:
Y1023 and Y1248 (Hazan et al. 1990);
Y1139, Y1221 and Y1248 (Ricci et al. 1995);
Y1248 (Pickl and Ries 2009);
Y1139 and Y1248 (Maadi et al. 2018) – this study also identified Y1005, Y1112, Y1127 and Y1196 as trans-autophosphorylation sites.
For ERBB2 homodimers, six canonical sites (Y1023, Y1139, Y1196, Y1221, Y1222 and Y1248) have been annotated as trans-autophosphorylation sites. This information will be revised as more experimental data becomes available.