Reactome | CA4:Zn2+ hydrates CO2 to HCO3-

CA4:Zn2+ hydrates CO2 to HCO3-

Stable Identifier

R-HSA-1237047

Type

Reaction [transition]

Species

Homo sapiens

Compartment

extracellular region, plasma membrane

Synonyms

Carbonic anhydrase IV hydrates carbon dioxide to bicarbonate and a proton, H2O + CO2 -> H+ + HCO3-

ReviewStatus

5/5

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Carbonic anhydrase IV (CA4) anchored to extracellular face of the plasma membrane (Wistrand et al. 1999) hydrates carbon dioxide (CO2) to yield bicarbonate (HCO3-) and a proton (H+) (Zhu & Sly 1990, Okayuma et al. 1992, Baird et al. 1997, Innocenti et al. 2004). During the reaction a hydroxyl group bound by the zinc ion (Zn2+) of CA4 attacks the CO2 molecule to directly form HCO3- (reviewed in Lindskog 1997). The HCO3- is displaced by water, which is then deprotonated by a histidine residue to recreate the Zn2+:hydroxyl group. Depending on the concentrations of reactants the reaction is reversible.

Literature References

PubMed ID	Title	Journal	Year
9054574	Catalysis and inhibition of human carbonic anhydrase IV Sly, WS, Fierke, CA, Okuyama, T, Baird TT, Jr, Waheed, A	Biochemistry	1997
2111324	Carbonic anhydrase IV from human lung. Purification, characterization, and comparison with membrane carbonic anhydrase from human kidney Sly, WS, Zhu, XL	J Biol Chem	1990
15501038	Carbonic anhydrase inhibitors: inhibition of the membrane-bound human isozyme IV with anions Innocenti, A, Wurl, M, Supuran, CT, Scozzafava, A, Antel, J, Firnges, MA	Bioorg Med Chem Lett	2004
1311094	Human carbonic anhydrase IV: cDNA cloning, sequence comparison, and expression in COS cell membranes Sly, WS, Sato, S, Okuyama, T, Waheed, A, Zhu, XL	Proc Natl Acad Sci U S A	1992
10090333	Carbonic anhydrase IV activity is localized on the exterior surface of human erythrocytes Carter, ND, Wistrand, PJ, Conroy, CW, Mahieu, I	Acta Physiol Scand	1999
9336012	Structure and mechanism of carbonic anhydrase Lindskog, S	Pharmacol Ther	1997