ALKBH3, a homolog of E.coli AlkB (Trewick et al. 2002), removes the methyl group from 1-methyladenine (1-meA) in a reaction dependent on alpha-ketoglutarate, oxygen and Fe2+. ALKBH3 directly reverses alkylating damage of DNA in the form of 1-meA that is accompanied with the release of formaldehyde (Duncan et al. 2002). The reversal of alkylating damage of dsDNA by ALKBH3 requires the presence of DNA helicase ASCC3, a component of the activating signal co-integrator complex (Dango et al. 2011). ALKBH3 can also repair methylated RNA (Aas et al. 2003).
Lindahl, T, Henshaw, TF, Hausinger, RP, Sedgwick, B, Trewick, SC
Falnes, PO, Otterlei, M, Seeberg, E, Akbari, M, Slupphaug, G, Vågbø, CB, Bjørås, M, Skorpen, F, Aas, PA, Sundheim, O, Krokan, HE
Xiong, LJ, Park, K, Mosammaparast, N, Dango, S, Shi, Y, Gygi, S, Sowa, ME, Harper, JW, Wu, F, Rubin, M
Lindahl, T, Duncan, T, Bates, PA, Sedgwick, B, Trewick, SC, Koivisto, P
DNA-N1-methyladenine dioxygenase activity of ALKBH3:Fe2+:ASCC1:ASCC2:ASCC3:1-meA-dsDNA [nucleoplasm]
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