ALKBH2 catalyzes the removal of the methyl group from 1-methyladenine (1-meA) in a reaction that depends on oxygen, alpha-ketoglutarate and Fe2+. ALKBH2 thus directly reverses alkylation damage of DNA in the form of 1-meA, releasing formaldehyde. ALKBH2 is ~4-fold more active on dsDNA containing 1-methyladenine than 3-methylcytosine (Duncan et al. 2002).
DNA-N1-methyladenine dioxygenase activity of ALKBH2:Fe2+:1-meA-dsDNA [nucleoplasm]