Reactome | Phospho-cPLA2 translocates to membranes when intracellular calcium levels increase

Phospho-cPLA2 translocates to membranes when intracellular calcium levels increase

Stable Identifier

R-HSA-111881

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, endoplasmic reticulum membrane

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

Signal Transduction (Homo sapiens)

- Signaling by GPCR (Homo sapiens)
  - GPCR downstream signalling (Homo sapiens)
    - G alpha (i) signalling events (Homo sapiens)
      - Opioid Signalling (Homo sapiens)
        
        G-protein mediated events (Homo sapiens)
        
        PLC beta mediated events (Homo sapiens)
        
        Ca-dependent events (Homo sapiens)
        
        phospho-PLA2 pathway (Homo sapiens)
        
        Phospho-cPLA2 translocates to membranes when intracellular calcium levels increase (Homo sapiens)

General

SBML | | PDF

SVG | | PPTX | SBGN

Phospho-cPLA2 translocates to membranes when intracellular calcium levels increase

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The 85kDa cytosolic phospholipase A2 (cPLA2 - PLA2G4A) is involved in cell signalling processes and inflammatory response and is regulated by phosphorylation and calcium concentrations. cPLA2 is phosphorylated at Ser727 and by a MAPK at Ser505. When phosphorylation is coupled with an influx of calcium ions, PLA2 becomes stimulated and translocates to the membrane where it releases arachidonate (AA) from membrane phospholipids. Calcium does not itself activate cPLA2. cPLA2 contains an N-terminal calcium-dependent phospholipid binding domain (CaLB) which shares homology with C2 domains (plays roles in signal transduction and membrane trafficking) and binds it to the membrane. Arachidonate is both a signalling molecule and the precursor for other signalling molecules termed eicosanoids (e.g., prostaglandins, leukotrienes and platelet-activating factor). A strict regulation of the activity of phospholipase enzyme is essential.

Literature References

PubMed ID	Title	Journal	Year
1904318	A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP Ramesha, CS, Kriz, RW, Knopf, JL, Lin, AY, Lin, LL, Clark, JD, Milona, N, Sultzman, LA	Cell	1991
9468497	Identification of the phosphorylation sites of cytosolic phospholipase A2 in agonist-stimulated human platelets and HeLa cells Dudler, T, Bartoli, F, Gelb, MH, Watson, SP, Kramer, RM, Apitz-Castro, R, Asselin, J, Börsch-Haubold, AG	J Biol Chem	1998
11375391	Intracellular calcium signals regulating cytosolic phospholipase A2 translocation to internal membranes Spencer, DM, Evans, JH, Zweifach, A, Leslie, CC	J Biol Chem	2001