FEN1 bound to PCNA and APEX1 cleaves flap ssDNA

Stable Identifier
R-HSA-110363
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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PCNA-bound FEN1 acts as a flap endonuclease to cleave the displaced single strand DNA (flap) containing the oxidatively damaged AP (apurinic/apyrimidinic) dideoxyribose residue (5'ddRP) that could not be excised by POLB. Interaction between FEN1 and PCNA or FEN1 and APE1 facilitate cleavage of flap structures (Klungland and Lindahl 1997, Matsumoto et al. 1999, Dianov et al. 1999).

Literature References
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Catalyst Activity
Catalyst Activity
Title
5'-flap endonuclease activity of APEX1:FEN1:PCNA:POLD,POLE:RPA:RFC:SSB(3'poly-dNMP-displaced 5'ddRP flap)-dsDNA [nucleoplasm]
Physical Entity
Activity
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