Reactome | POLH binds monoUb:K164-PCNA at damaged TT-CPD-DNA template

POLH binds monoUb:K164-PCNA at damaged TT-CPD-DNA template

Stable Identifier

R-HSA-110316

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

Synonyms

Binding of Pol eta to lesioned DNA template

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POLH binds monoUb:K164-PCNA at damaged TT-CPD-DNA template

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DNA polymerase eta (POLH) belongs to Y family of DNA polymerases. POLH binds PCNA monoubiquitinated at lysine K164 by the RAD18:UBE2B (RAD18:RAD6) or RBX1:CUL4:DDB1:DTL complexes in response to DNA damage. POLH C-terminus contains a conserved PCNA interaction motif, while the catalytic domain of POLH contains a conserved monoubiquitin binding motif. POLH is most efficient in recognition and repair of thymine-thymine cyclobutane pyrimidine dimers (TT-CPD) induced by UV-mediated DNA damage (Masutani et al. 2000, Kannouche et al. 2004)

Literature References

PubMed ID	Title	Journal	Year
15149598	Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage Kannouche, PL, Wing, J, Lehmann, AR	Mol. Cell	2004
10856253	Mechanisms of accurate translesion synthesis by human DNA polymerase eta. Masutani, C, Kusumoto, R, Iwai, S, Hanaoka, F	EMBO J	2000