Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase

Stable Identifier
R-HSA-110229
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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NTHL1 (hNTH1; endonuclease III-like protein 1) acts as a FapyA DNA glycosylase to cleave FapyA (4,6-diamino-5-formamidopyrimidine), an imidazole ring-opened adenine derivative formed during oxidative stress (Evans et al. 2004), from the DNA sugar phosphate backbone, creating an apurinic/apyrimidinic (AP) site (Luna et al. 2000, Hu et al. 2005). After the AP site is created, NTHL1 can act as a beta lyase to cleave the DNA strand 5' to the AP site (Ikeda et al. 1998).

Literature References
PubMed ID Title Journal Year
15341901 Oxidative DNA damage and disease: induction, repair and significance

Dizdaroglu, M, Cooke, MS, Evans, MD

Mutat. Res. 2004
10882850 Cell-cycle regulation, intracellular sorting and induced overexpression of the human NTH1 DNA glycosylase involved in removal of formamidopyrimidine residues from DNA

Luna, L, Hoff, E, Bjørås, M, Rognes, T, Seeberg, E

Mutat. Res. 2000
16221681 Repair of formamidopyrimidines in DNA involves different glycosylases: role of the OGG1, NTH1, and NEIL1 enzymes

Dizdaroglu, M, Hogue, BA, Bohr, VA, de Souza-Pinto, NC, Jaruga, P, Haraguchi, K, Greenberg, MM, Hu, J

J. Biol. Chem. 2005
9705289 Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue

Seki, S, Roy, R, Izumi, T, Ikeda, S, Kurosky, A, Sarker, AH, Biswas, T, Boldogh, I, Mitra, S

J. Biol. Chem. 1998
Participants
Participates
Catalyst Activity

oxidized purine nucleobase lesion DNA N-glycosylase activity of NTHL1:FapyA-dsDNA [nucleoplasm]

Orthologous Events
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