Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase

Stable Identifier
Reaction [transition]
Homo sapiens
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NTHL1 (hNTH1; endonuclease III-like protein 1) acts as a FapyA DNA glycosylase to cleave FapyA (4,6-diamino-5-formamidopyrimidine), an imidazole ring-opened adenine derivative formed during oxidative stress (Evans et al. 2004), from the DNA sugar phosphate backbone, creating an apurinic/apyrimidinic (AP) site (Luna et al. 2000, Hu et al. 2005). After the AP site is created, NTHL1 can act as a beta lyase to cleave the DNA strand 5' to the AP site (Ikeda et al. 1998).
Literature References
PubMed ID Title Journal Year
16221681 Repair of formamidopyrimidines in DNA involves different glycosylases: role of the OGG1, NTH1, and NEIL1 enzymes

Dizdaroglu, M, Hogue, BA, Bohr, VA, de Souza-Pinto, NC, Jaruga, P, Haraguchi, K, Greenberg, MM, Hu, J

J. Biol. Chem. 2005
9705289 Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue

Seki, S, Roy, R, Izumi, T, Ikeda, S, Kurosky, A, Sarker, AH, Biswas, T, Boldogh, I, Mitra, S

J. Biol. Chem. 1998
10882850 Cell-cycle regulation, intracellular sorting and induced overexpression of the human NTH1 DNA glycosylase involved in removal of formamidopyrimidine residues from DNA

Luna, L, Hoff, E, Bjørås, M, Rognes, T, Seeberg, E

Mutat. Res. 2000
15341901 Oxidative DNA damage and disease: induction, repair and significance

Dizdaroglu, M, Cooke, MS, Evans, MD

Mutat. Res. 2004
Catalyst Activity

oxidized purine nucleobase lesion DNA N-glycosylase activity of NTHL1:FapyA-dsDNA [nucleoplasm]

Orthologous Events
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