Reactome | Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase

Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase

Stable Identifier

R-HSA-110229

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase

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NTHL1 (hNTH1; endonuclease III-like protein 1) acts as a FapyA DNA glycosylase to cleave FapyA (4,6-diamino-5-formamidopyrimidine), an imidazole ring-opened adenine derivative formed during oxidative stress (Evans et al. 2004), from the DNA sugar phosphate backbone, creating an apurinic/apyrimidinic (AP) site (Luna et al. 2000, Hu et al. 2005). After the AP site is created, NTHL1 can act as a beta lyase to cleave the DNA strand 5' to the AP site (Ikeda et al. 1998).

Literature References

PubMed ID	Title	Journal	Year
16221681	Repair of formamidopyrimidines in DNA involves different glycosylases: role of the OGG1, NTH1, and NEIL1 enzymes Hu, J, de Souza-Pinto, NC, Haraguchi, K, Hogue, BA, Jaruga, P, Greenberg, MM, Dizdaroglu, M, Bohr, VA	J. Biol. Chem.	2005
9705289	Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue Ikeda, S, Biswas, T, Roy, R, Izumi, T, Boldogh, I, Kurosky, A, Sarker, AH, Seki, S, Mitra, S	J. Biol. Chem.	1998
10882850	Cell-cycle regulation, intracellular sorting and induced overexpression of the human NTH1 DNA glycosylase involved in removal of formamidopyrimidine residues from DNA Luna, L, Bjørås, M, Hoff, E, Rognes, T, Seeberg, E	Mutat. Res.	2000
15341901	Oxidative DNA damage and disease: induction, repair and significance Evans, MD, Dizdaroglu, M, Cooke, MS	Mutat. Res.	2004