Reactome | Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase

Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase

Stable Identifier

R-HSA-110229

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

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Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase

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NTHL1 (hNTH1; endonuclease III-like protein 1) acts as a FapyA DNA glycosylase to cleave FapyA (4,6-diamino-5-formamidopyrimidine), an imidazole ring-opened adenine derivative formed during oxidative stress (Evans et al. 2004), from the DNA sugar phosphate backbone, creating an apurinic/apyrimidinic (AP) site (Luna et al. 2000, Hu et al. 2005). After the AP site is created, NTHL1 can act as a beta lyase to cleave the DNA strand 5' to the AP site (Ikeda et al. 1998).

Literature References

PubMed ID	Title	Journal	Year
9705289	Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue Ikeda, S, Biswas, T, Roy, R, Izumi, T, Boldogh, I, Kurosky, A, Sarker, AH, Seki, S, Mitra, S	J. Biol. Chem.	1998
16221681	Repair of formamidopyrimidines in DNA involves different glycosylases: role of the OGG1, NTH1, and NEIL1 enzymes Hu, J, de Souza-Pinto, NC, Haraguchi, K, Hogue, BA, Jaruga, P, Greenberg, MM, Dizdaroglu, M, Bohr, VA	J. Biol. Chem.	2005
10882850	Cell-cycle regulation, intracellular sorting and induced overexpression of the human NTH1 DNA glycosylase involved in removal of formamidopyrimidine residues from DNA Luna, L, Bjørås, M, Hoff, E, Rognes, T, Seeberg, E	Mutat. Res.	2000
15341901	Oxidative DNA damage and disease: induction, repair and significance Evans, MD, Dizdaroglu, M, Cooke, MS	Mutat. Res.	2004