Cleavage of thymine glycol by NTHL1 glycosylase

Stable Identifier
R-HSA-110224
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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NTHL1 (hNTH1; endonuclease III-like protein 1) cleaves oxidized thymine in the form of thymine glycol from DNA sugar-phosphate backbone and acts as a beta lyase to cleave the DNA sugar-phosphate backbone 5' to the apurinic/apyrimidinic (AP) site generated in the glycolysis step (Ikeda et al. 1998, Dizdaroglu et al. 1999, Miyabe et al. 2002).

Literature References
PubMed ID Title Journal Year
12140329 Identification of 5-formyluracil DNA glycosylase activity of human hNTH1 protein

Miyabe, I, Zhang, QM, Kino, K, Sugiyama, H, Takao, M, Yasui, A, Yonei, S

Nucleic Acids Res. 2002
9705289 Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue

Ikeda, S, Biswas, T, Roy, R, Izumi, T, Boldogh, I, Kurosky, A, Sarker, AH, Seki, S, Mitra, S

J. Biol. Chem. 1998
9890904 Excision of products of oxidative DNA base damage by human NTH1 protein

Dizdaroglu, M, Karahalil, B, Sentürker, S, Buckley, TJ, Roldán-Arjona, T

Biochemistry 1999
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity of NTHL1:Tg-dsDNA [nucleoplasm]
Physical Entity
Activity
Orthologous Events
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