Reactome | Cleavage of uracil by UNG glycosylase

Cleavage of uracil by UNG glycosylase

Stable Identifier

R-HSA-110215

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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UNG, a DNA uracil glycosylase, excises the uracil base generated when a DNA damaging agent deaminates cytosine (creating a U:G base pair) or when dUMP is misincorporated during DNA synthesis (creating a U:A base pair). UNG scans the DNA for damage by kinking and compressing the DNA phosphate backbone with a serine-proline pinch, which causes uracil to flip out at the phosphate-sugar junction into the recognition pocket of the UNG. The subsequent excision of uracil creates an apurinic/apyrimidinic (AP) site in the DNA (Parikh et al. 1998).

Literature References

PubMed ID	Title	Journal	Year
9724657	Base excision repair initiation revealed by crystal structures and binding kinetics of human uracil-DNA glycosylase with DNA Tainer, JA, Bharati, S, Krokan, HE, Mol, CD, Slupphaug, G, Parikh, SS	EMBO J	1998