Cleavage of uracil by UNG glycosylase

Stable Identifier
R-HSA-110215
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
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UNG, a DNA uracil glycosylase, excises the uracil base generated when a DNA damaging agent deaminates cytosine (creating a U:G base pair) or when dUMP is misincorporated during DNA synthesis (creating a U:A base pair). UNG scans the DNA for damage by kinking and compressing the DNA phosphate backbone with a serine-proline pinch, which causes uracil to flip out at the phosphate-sugar junction into the recognition pocket of the UNG. The subsequent excision of uracil creates an apurinic/apyrimidinic (AP) site in the DNA (Parikh et al. 1998).

Literature References
PubMed ID Title Journal Year
9724657 Base excision repair initiation revealed by crystal structures and binding kinetics of human uracil-DNA glycosylase with DNA

Parikh, SS, Mol, CD, Slupphaug, G, Bharati, S, Krokan, HE, Tainer, JA

EMBO J 1998
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
uracil DNA N-glycosylase activity of UNG-1:(Ura:Gua)-dsDNA [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created
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