TMP, uridine 2', 3', or 5' monophosphates, or deoxyuridine 3' or 5' monophosphates + H2O => thymidine or (deoxy)uridine + orthophosphate [NT5C]

Stable Identifier
Reaction [transition]
Homo sapiens
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Cytosolic 5'3'-nucleotidase (NT5C) catalyzes the hydrolysis of uridine 2', 3', and 5' monophosphates, dexoyuridine 3' and 5' monophosphates, and thymidine monophosphate to yield the corresponding (deoxy)nucleosides and orthophosphate. The active form of the enzyme is a homodimer, with an absolute requirement for Mg++ (Hoglund and Reichard 1990; Rampazzo et al. 2000). This enzyme appears to play a central role in the "substrate cycles" that regulate cytosolic deoxynucleotide levels (Gazziola et al. 2001).

Literature References
PubMed ID Title Journal Year
2157703 Cytoplasmic 5'(3')-nucleotidase from human placenta

Hoglund, L, Reichard, PA

J Biol Chem 1990
11083867 Cytosolic high Km 5'-nucleotidase and 5'(3')-deoxyribonucleotidase in substrate cycles involved in nucleotide metabolism

Bianchi, V, Moras, M, Reichard, PA, Ferraro, P, Gazziola, C

J Biol Chem 2001
10681516 Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells

Bianchi, V, Reichard, PA, Karlsson, A, Ferraro, P, Gallinaro, L, Hellman, U, Rampazzo, C, Johansson, M

J Biol Chem 2000
Catalyst Activity

5'-nucleotidase activity of NT5C dimer [cytosol]

Orthologous Events
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