Reactome | TMP, uridine 2', 3', or 5' monophosphates, or deoxyuridine 3' or 5' monophosphates + H2O => thymidine or (deoxy)uridine + orthophosphate [NT5C]

TMP, uridine 2', 3', or 5' monophosphates, or deoxyuridine 3' or 5' monophosphates + H2O => thymidine or (deoxy)uridine + orthophosphate [NT5C]

Stable Identifier

R-HSA-109480

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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TMP, uridine 2', 3', or 5' monophosphates, or deoxyuridine 3' or 5' monophosphates + H2O => thymidine or (deoxy)uridine + orthophosphate [NT5C]

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Cytosolic 5'3'-nucleotidase (NT5C) catalyzes the hydrolysis of uridine 2', 3', and 5' monophosphates, dexoyuridine 3' and 5' monophosphates, and thymidine monophosphate to yield the corresponding (deoxy)nucleosides and orthophosphate. The active form of the enzyme is a homodimer, with an absolute requirement for Mg++ (Hoglund and Reichard 1990; Rampazzo et al. 2000). This enzyme appears to play a central role in the "substrate cycles" that regulate cytosolic deoxynucleotide levels (Gazziola et al. 2001).

Literature References

PubMed ID	Title	Journal	Year
2157703	Cytoplasmic 5'(3')-nucleotidase from human placenta Hoglund, L, Reichard, PA	J Biol Chem	1990
11083867	Cytosolic high Km 5'-nucleotidase and 5'(3')-deoxyribonucleotidase in substrate cycles involved in nucleotide metabolism Gazziola, C, Ferraro, P, Moras, M, Reichard, PA, Bianchi, V	J Biol Chem	2001
10681516	Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells Rampazzo, C, Johansson, M, Gallinaro, L, Ferraro, P, Hellman, U, Karlsson, A, Reichard, PA, Bianchi, V	J Biol Chem	2000