(d)CMP, TMP, or (d)UMP + H2O => (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C1A)

Stable Identifier
Reaction [transition]
Homo sapiens
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Cytosolic 5'-nucleotidase IA (NT5C1A) catalyzes the hydrolysis of (deoxy)cytidine monophosphate, thymidine monophosphate and (deoxy)uridine monophosphate to the corresponding nucleosides plus orthophosphate. The enzyme is allosterically activated by ADP (Hunsucker et al. 2001). The human enzyme is inferred to be a homotetramer with one Mg++ ion bound per subunit based on its similarity to the pigeon heart enzyme (Bianchi and Spychala 2003; Sala-Newby et al. 1999; Skladanowski and Newby 1990).

Literature References
PubMed ID Title Journal Year
11133996 Human cytosolic 5'-nucleotidase I. Characterization and role in nucleoside analog resistance

Hunsucker, SA, Spychala, J, Mitchell, BS

J Biol Chem 2001
10364222 The mechanism of adenosine formation in cells. Cloning of cytosolic 5'-nucleotidase-I

Skladanowski, A, Newby, AC, Sala-Newby, GB

J Biol Chem 1999
12947102 Mammalian 5'-nucleotidases

Spychala, J, Bianchi, V

J Biol Chem 2003
2344353 Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart

Skladanowski, A, Newby, AC

Biochem J 1990
Catalyst Activity

5'-nucleotidase activity of NT5C1A tetramer [cytosol]

This event is regulated
Positively by