TDG glycosylase mediated recognition and binding of thymine opposite guanine

Stable Identifier
R-GGA-353426
Type
Reaction [transition]
Species
Gallus gallus
Compartment
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The G/T mismatch specific thymine DNA glycosylase (EC 3.2.2.-) removes the mismatched T with opposite a G residue by its DNA N-glycosylase activity. It is one of the essential components of a functional DNA demethylation complex in chicken embryos, and which also contains 5-methylcytosine DNA glycosylase (5-MCDG), RNA helicase, and G/T mismatch DNA glycosylase activities. The chicken homologue of human G/T mismatch DNA glycosylase shows 80% identity with the human G/T mismatch DNA glycosylase, and both the C- and N-terminal parts have about 50% identity. The recombinant chicken protein expressed in Escherichia coli has both G/T mismatch and 5-MCDG activities. Hemimethylated DNA is a better substrate than symmetrically methylated CpGs for the 5-MCDG activity. Deletion mutations indicate that G/T mismatch and 5-MCDG activities require the same areas of the N- and C-terminal parts of the protein.

Literature References
PubMed ID Title Journal Year
11058112 5-Methylcytosine DNA glycosylase activity is also present in the human MBD4 (G/T mismatch glycosylase) and in a related avian sequence

Zhu, B, Zheng, Y, Angliker, H, Schwarz, S, Thiry, S, Siegmann, M, Jost, JP

Nucleic Acids Res 2000
10779566 5-methylcytosine-DNA glycosylase activity is present in a cloned G/T mismatch DNA glycosylase associated with the chicken embryo DNA demethylation complex

Zhu, B, Zheng, Y, Hess, D, Angliker, H, Schwarz, S, Siegmann, M, Thiry, S, Jost, JP

Proc Natl Acad Sci U S A 2000
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Catalyst Activity
Title
DNA N-glycosylase activity of TDG [nucleoplasm]
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