Non-homologous end joining (NHEJ)

Stable Identifier
R-GGA-353423
Type
Pathway
Species
Gallus gallus
Compartment
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The NHEJ pathway is initiated in response to the formation of a DNA double-strand break (DSB) induced by a DNA-damaging agent such as ionizing radiation. First, the Ku70/80 heterodimer binds to the ends of the DSB. The catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs) is then recruited to DNA-bound Ku to form the DNA-PK holoenzyme. The ends of the break are brought together as two molecules of DNA-PK (one at each end of the break) are joined in a synaptic complex. Following the formation of the synaptic complex, the XRCC4/DNA ligase IV complex is recruited. Prior to end rejoining by ligation, repair protein factors must be removed from the DNA. Both Mg-ATP and the protein kinase activity of DNA-PKcs are required for NHEJ. NHEJ has been suggested as the main pathway for rejoining of IR-induced DSBs in vertebrates. The involvement of homologous recombination repair (HRR) has been postulated to occur only after initial rejoining by NHEJ.

Literature References
PubMed ID Title Journal Year
16807135 Differential usage of non-homologous end-joining and homologous recombination in double strand break repair

Sonoda, E, Hochegger, H, Saberi, A, Taniguchi, Y, Takeda, S

DNA Repair (Amst) 2006
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