Removal of repair proteins and ligation of the processed ends of the DNA double-strand break

Stable Identifier
Reaction [transition]
Gallus gallus
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Just before final end joining, the bound repair proteins must be released. However, the precise steps by which this happens is not known. The repair of DSB is an energy consuming event that requires DNA PK kinase activity as well (Malu et al. 2012, Hsu et al. 2002, Lee et al. 2004, Nick McElhinny et al. 2000).

Literature References
PubMed ID Title Journal Year
22529269 Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs

Malu, S, De Ioannes, P, Kozlov, M, Greene, M, Francis, D, Hanna, M, Pena, J, Escalante, CR, Kurosawa, A, Erdjument-Bromage, H, Tempst, P, Adachi, N, Vezzoni, P, Villa, A, Aggarwal, AK, Cortes, P

J. Exp. Med. 2012
18046455 Crystal structure of human XLF/Cernunnos reveals unexpected differences from XRCC4 with implications for NHEJ

Li, Y, Chirgadze, DY, Bolanos-Garcia, VM, Sibanda, BL, Davies, OR, Ahnesorg, P, Jackson, SP, Blundell, TL

EMBO J. 2008
12509254 Defining interactions between DNA-PK and ligase IV/XRCC4

Hsu, HL, Yannone, SM, Chen, DJ

DNA Repair (Amst.) 2002
10757784 Ku recruits the XRCC4-ligase IV complex to DNA ends

Nick McElhinny, SA, Snowden, CM, McCarville, J, Ramsden, DA

Mol. Cell. Biol. 2000
14561766 Implication of DNA polymerase lambda in alignment-based gap filling for nonhomologous DNA end joining in human nuclear extracts

Lee, JW, Blanco, L, Zhou, T, Garcia-Diaz, M, Bebenek, K, Kunkel, TA, Wang, Z, Povirk, LF

J. Biol. Chem. 2004
Participant Of
Catalyst Activity
Catalyst Activity
DNA ligase activity of DNA-PK:XRCC4:DNA ligase IV:DNA complex associated with ligatable DNA ends [nucleoplasm]
Physical Entity