Association of DNA-PKcs with Ku-bound ends of DNA double-strand breaks

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Gallus gallus
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Non homologous end joining (NHEJ) requires DNA dependent protein kinase (DNA-PK) for DSB repair. DNA PK is comprised by a catalytic subunit (DNA-PKcs) and a heterodimer of the Ku70 and Ku80 DNA-binding regulatory subunits. DNA-PKcs is recruited to the complex of Ku bound to the DNA end, causing Ku to translocate inwards (away from the break). This forms the DNA-PK/Ku complex (DNA-PKcs plus Ku70/Ku80) at each end of the DNA double strand break. Ku and DNA-PKcs play essential roles in double stranded DNA break repair and V(D)J recombination and have been implicated in the transcriptional regulation of specific genes. Ku functions as a docking module for homeodomain proteins, allowing phosphorylation by DNA-PK. The Ku complex has also been implicated as an inhibitory factor for HR-mediated DNA repair, perhaps by competing with factors involved in HR for DSB recognition.

Literature References
PubMed ID Title Journal Year
11279128 The binding of Ku antigen to homeodomain proteins promotes their phosphorylation by DNA-dependent protein kinase

Schild-Poulter, C, Pope, L, Giffin, W, Kochan, JC, Ngsee, JK, Traykova-Andonova, M, Haché, RJ

J Biol Chem 2001
13678345 DNA-PK: the major target for wortmannin-mediated radiosensitization by the inhibition of DSB repair via NHEJ pathway

Hashimoto, M, Rao, S, Tokuno, O, Yamamoto, K, Takata, M, Takeda, S, Utsumi, H

J Radiat Res (Tokyo) 2003
11577093 Genetic analysis of the DNA-dependent protein kinase reveals an inhibitory role of Ku in late S-G2 phase DNA double-strand break repair

Fukushima, T, Takata, M, Morrison, C, Araki, R, Fujimori, A, Abe, M, Tatsumi, K, Jasin, M, Dhar, PK, Sonoda, E, Chiba, T, Takeda, S

J Biol Chem 2001
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