Cytosolic enolase catalyzes the reversible magnesium-dependent hydration of phosphoenolpyruvate to form 2-phosphoglycerate. The active form of the enzyme is a dimer. Three enolase isoforms have been isolated and biochemically characterized, 1 (alpha), 2 (gamma), and 3 (beta), as have 1:1, 1:2, 2:2, and 3:3 enolase dimers (Russell et al. 1986; Tanaka et al. 1985, 1995, 1998).
phosphopyruvate hydratase activity of enolase dimer [cytosol]