Rad51 assembly at the DNA-damage sites on the chromatin

Stable Identifier
R-GGA-265940
Type
Reaction [transition]
Species
Gallus gallus
Compartment
nucleoplasm
ReviewStatus
5/5
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Rad51 assembly at the DNA-damage sites on the chromatin
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Homologous recombination in vertebrates is catalyzed by Rad51. Like bacterial RecA, Rad51 polymerizes on single stranded DNA to form nucleoprotein filaments that catalyze pairing and strand exchange with homologous DNA. Two breast cancer susceptible genes seem to regulate this event either directly or indirectly. BRCA1 contributes to DNA repair by regulating recombinase Rad51 assembly in a manner functionally analogous to various Rad51 assembly factors and Rad51 mediators. BRCA1 is also involved in correct localization of BRCA2 to chromatin at damage foci. CHK2 mediated BRCA1 phosphorylation is also required for Rad51 assembly. BRCA2 is a Rad51 mediator that acts to displace RPA and load Rad51 onto ssDNA.
Literature References
PubMed ID Title Journal Year
16391231 Multifactorial contributions to an acute DNA damage response by BRCA1/BARD1-containing complexes

Livingston, DM, Cantor, SB, Sobhian, B, Greenberg, RA, Nakatani, Y, Pathania, S

Genes Dev 2006
9008167 Association of BRCA1 with Rad51 in mitotic and meiotic cells

Livingston, DM, Scully, R, Feunteun, J, Xiao, Y, Plug, A, Chen, J, Ashley, T, Weaver, D

Cell 1997
15800615 CDK-dependent phosphorylation of BRCA2 as a regulatory mechanism for recombinational repair

West, SC, Liu, Y, Esashi, F, Hunt, T, Gannon, J, Christ, N, Jasin, M

Nature 2005
10197592 BRCA1, BRCA2, and Rad51 operate in a common DNA damage response pathway

Livingston, DM, Chen, JJ, Scully, R, Silver, D, Cantor, S

Cancer Res 1999
11100717 In search of the tumour-suppressor functions of BRCA1 and BRCA2

Livingston, DM, Scully, R

Nature 2000
17515904 Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2

West, SC, Egelman, EH, Galkin, VE, Esashi, F, Yu, X

Nat Struct Mol Biol 2007
9430650 Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death

Bezzubova, O, Takeda, S, Takata, M, Buerstedde, JM, Yamaguchi-Iwai, Y, Sasaki, MS, Ogawa, H, Sonoda, E, Shinohara, A

EMBO J 1998
Participants
Input
Output
Participates
as an event of
This event is regulated
Positively by
Regulator
BRCA1 [nucleoplasm] (Gallus gallus)
Regulator
BRCA2 [nucleoplasm] (Gallus gallus)
Authored
Saxena, A  (2008-01-04)
Reviewed
Borowiec, JA  (2009-04-24)
Created
Saxena, A  (2008-04-21)
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