Serrate binds Notch in cis

Stable Identifier
R-DME-2076706
Type
Reaction [binding]
Species
Drosophila melanogaster
Compartment
General
SVG |   | PPTX  | SBGN
Serrate binds Notch in cis

Wild-type Serrate constructs and mutant Serrate constructs, harboring mutations in surface exposed amino acid residues of the DSL domain, were expressed in fly cultures along the anterior-posterior compartment boundary of the third instar Drosophila imaginal wing disc. The ability of each construct to induce Notch signaling was assayed through ectopic expression of wingless, a reporter gene for Notch activity, and by the ability of each construct to induce ectopic wing margin or to suppress the normal margin of the adult wing. Cis-interactions blocked both ectopic Notch signaling and the endogenous Notch activity present at the dorsal-ventral boundary of the wing disc. Mutations in the surface exposed amino-acid residues of the DSL domain of Serrate affected both cis- and trans-interactions, indicating that the surface exposed residues of the DSL domain are involved in Notch binding. Mutation of the highly conserved phenylalanine residue F257 completely abrogated both trans-activation and cis-inhibition.

Literature References
PubMed ID Title Journal Year
18660822 A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition

Cordle, J, Johnson, S, Tay, JZ, Roversi, P, Wilkin, MB, Hernandez de Madrid Diaz, B, Shimizu, H, Jensen, S, Whiteman, P, Jin, B, Redfield, C, Baron, M, Lea, SM, Handford, PA

Nat Struct Mol Biol 2008
Participants
Orthologous Events
Authored
Reviewed
Created