NR5A2, ZNF217 and NCOA3 bind GREB1 promoter

Stable Identifier
R-HSA-9012000
Type
Reaction [omitted]
Species
Homo sapiens
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GREB1 (growth regulation by estrogen in breast cancer 1) is an estrogen-responsive gene that contains three EREs located 1.6, 9.5 and 21.2 kb upstream of the transcriptional start site (Ghosh et al 2000; Lin et al, 2004; Rae et al, 2005; Deschenes et al, 2007; Sun et al, 2007). By ChIP, all three EREs are bound by ESR1 and the transcriptional co-activator NCOA3 (also known as SRC3). Although this binding occurs even in the absence of estradiol treatment, binding is enhanced after estrogen stimulation (Sun et al, 2007). Estrogen-dependent GREB1 expression also depends on removal of the repressive H3K9 methlyation mark by KDM4B (Kawazu et al, 2011; Gaughan et al, 2013). Estrogen stimulation increases the occupancy of RNA polymerase II at the GREB1 gene and may promote transcription through the formation of chromatin loops. Estrogen stimulation also increases the level of H4 acetylation at the promoter (Sun et al, 2007; Deschenes et al, 2007). In addition to ESR1 and NCOA3, Kruppel-like finger (KLF) protein ZNF217 has also been shown to bind to ESR1 and enhance recruitment to GREB1 EREs. ZNF217 overexpression is associated with anchorage independent growth in MCF7 cell lines (Nguyen et al, 2014). Although both NCOA3 and ZNF217 have been shown to interact with the GREB1 EREs, no study has examined co-occupancy of the GREB1 enhancer by these two regulators.

Literature References
PubMed ID Title Journal Year
15345050 Discovery of estrogen receptor alpha target genes and response elements in breast tumor cells

Lin, CY, Ström, A, Vega, VB, Kong, SL, Yeo, AL, Thomsen, JS, Chan, WC, Doray, B, Bangarusamy, DK, Ramasamy, A, Vergara, LA, Tang, S, Chong, A, Bajic, VB, Miller, LD, Gustafsson, JA, Liu, ET

Genome Biol. 2004
24973012 A functional interplay between ZNF217 and estrogen receptor alpha exists in luminal breast cancers

Nguyen, NT, Vendrell, JA, Poulard, C, Győrffy, B, Goddard-Léon, S, Bièche, I, Corbo, L, Le Romancer, M, Bachelot, T, Treilleux, I, Cohen, PA

Mol Oncol 2014
15986123 GREB 1 is a critical regulator of hormone dependent breast cancer growth

Rae, JM, Johnson, MD, Scheys, JO, Cordero, KE, Larios, JM, Lippman, ME

Breast Cancer Res. Treat. 2005
11103799 PDZK1 and GREB1 are estrogen-regulated genes expressed in hormone-responsive breast cancer

Ghosh, MG, Thompson, DA, Weigel, RJ

Cancer Res. 2000
17666587 Long-range activation of GREB1 by estrogen receptor via three distal consensus estrogen-responsive elements in breast cancer cells

Sun, J, Nawaz, Z, Slingerland, JM

Mol. Endocrinol. 2007
23723241 KDM4B is a master regulator of the estrogen receptor signalling cascade

Gaughan, L, Stockley, J, Coffey, K, O'Neill, D, Jones, DL, Wade, M, Wright, J, Moore, M, Tse, S, Rogerson, L, Robson, CN

Nucleic Acids Res. 2013
17463000 Regulation of GREB1 transcription by estrogen receptor alpha through a multipartite enhancer spread over 20 kb of upstream flanking sequences

Deschênes, J, Bourdeau, V, White, JH, Mader, S

J. Biol. Chem. 2007
21445275 Histone demethylase JMJD2B functions as a co-factor of estrogen receptor in breast cancer proliferation and mammary gland development

Kawazu, M, Saso, K, Tong, KI, McQuire, T, Goto, K, Son, DO, Wakeham, A, Miyagishi, M, Mak, TW, Okada, H

PLoS ONE 2011
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