NEDD8-UBA3:NAE1 binds a second NEDD8

Stable Identifier
R-HSA-8951656
Type
Reaction [transition]
Species
Homo sapiens
Compartment
ReviewStatus
5/5
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After covalent attachment of the first NEDD8 molecule to the catalytic cysteine, a second NEDD8 binds to the now-free adenylation site on the UBA3 subunit of UBA3:NAE1. This 'doubly-loaded' E1 enzyme is primed to transfer the covalently-bound NEDD8 to the downstream E2 enzyme (Huang et al, 2005; Huang et al, 2007; reviewed in Enchev et al, 2015). Note that although not depicted here, the second NEDD8 moiety is covalently adenylated at its C-terminal glycine residue at the end of this reaction.
Literature References
PubMed ID Title Journal Year
17220875 Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity

Huang, DT, Ohi, MD, Hunt, HW, Schulman, BA, Holton, JM, Zhuang, M

Nature 2007
15694336 Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1

Huang, DT, Schulman, BA, Holton, JM, Paydar, A, Waddell, MB, Zhuang, M

Mol. Cell 2005
25531226 Protein neddylation: beyond cullin-RING ligases

Schulman, BA, Enchev, RI, Peter, M

Nat. Rev. Mol. Cell Biol. 2015
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