Reactome | SHPRH polyubiquitinates monoubiquitinated PCNA

SHPRH polyubiquitinates monoubiquitinated PCNA

Stable Identifier

R-HSA-8943003

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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SHPRH polyubiquitinates monoubiquitinated PCNA

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In response to a stalled replication fork, SHPRH polyubiquitinates lysine-164 of PCNA that has already been monoubiquitinated on lysine-164 by RAD18:UBE2B (RAD18:RAD6) (Unk et al. 2006, Motegi et al. 2006, Motegi et al. 2008). The ubiquitin donor is the E2 complex UBE2N:UBE2V2 (UBC13:MMS2) containing ubiquitin conjugated to UBE2N. The resulting polyubiquitin chain contains lysine-63 (K63) linkages and appears to change the repair process from translesion synthesis (TLS) to template switching (TS). SHPRH interacts directly with PCNA, RAD18:UBE2B, and UBE2N:UBE2V2.

Literature References

PubMed ID	Title	Journal	Year
18719106	Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks Roest, HP, Ding, H, Motegi, A, Myung, K, Markowitz, SD, Wu, X, Liaw, HJ, Lee, KY, Moinova, H, Hoeijmakers, JH, Maas, A	Proc. Natl. Acad. Sci. U.S.A.	2008
17108083	Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen Haracska, L, Hurwitz, J, Blastyák, A, Bermudez, V, Prakash, L, Fatyol, K, Hajdu, I, Unk, I, Szakál, B, Prakash, S	Proc. Natl. Acad. Sci. U.S.A.	2006
17130289	Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination Motegi, A, Myung, K, Markowitz, SD, Sood, R, Liu, PP, Moinova, H	J. Cell Biol.	2006