TPX2 binds AURKA at centrosomes

Stable Identifier
R-HSA-8853405
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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General
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TPX2 binds to aurora kinase A (AURKA) at centrosomes. The first 43 amino acids at the N-terminus of TPX2 are needed for binding to AURKA (Bayliss et al. 2003). HMMR (RHAMM) binds to TPX2 (Groen et al. 2004, Maxwell et al. 2005) and is involved in the proper localization of TPX2 to centrosomes and TPX2-mediated AURKA activation (Chen et al. 2014, Scrofani et al. 2015).

TPX2 binding to Aurora A protects premature AURKA degradation by APC/C-mediated proteolysis during early mitosis. TPX2 differentially regulates AURKA stability, activity and localization. While amino acids 1-43 in TPX2 facilitate complex formation between AURKA and TPX2 and promote kinase activation, they are insufficient for AURKA targeting to the mitotic spindle (Giubettini et al. 2011).

Literature References
PubMed ID Title Journal Year
15705883 Receptor for hyaluronan-mediated motility correlates with centrosome abnormalities in multiple myeloma and maintains mitotic integrity

Maxwell, CA, Keats, JJ, Belch, AR, Pilarski, LM, Reiman, T

Cancer Res. 2005
15498487 XRHAMM functions in ran-dependent microtubule nucleation and pole formation during anastral spindle assembly

Groen, AC, Cameron, LA, Coughlin, M, Miyamoto, DT, Mitchison, TJ, Ohi, R

Curr. Biol. 2004
25532896 Microtubule nucleation in mitosis by a RanGTP-dependent protein complex

Scrofani, J, Sardon, T, Meunier, S, Vernos, I

Curr. Biol. 2015
14580337 Structural basis of Aurora-A activation by TPX2 at the mitotic spindle

Bayliss, R, Sardon, T, Vernos, I, Conti, E

Mol. Cell 2003
Participants
Participant Of
Orthologous Events
Authored
Reviewed
Created