Reactome | DCLRE1C (ARTEMIS) processes DNA DSB ends

DCLRE1C (ARTEMIS) processes DNA DSB ends

Stable Identifier

R-HSA-5693533

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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DCLRE1C (ARTEMIS) processes DNA DSB ends

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DCLRE1C (ARTEMIS) possesses an intrinsic 5' to 3' exonuclease activity. Upon binding to PRKDC (DNA-PKcs) at DNA double strand breaks (DSBs) and undergoing PRKDC-mediated phosphorylation, DCLRE1C acquires endonucleolytic activity towards 5' and 3' overhangs at DNA double strand breaks, and it also acquires a hairpin opening activity (Ma et al. 2002, Ma et al. 2005). Autophosphorylation of PRKDC, although not required for the kinase activity of PRKDC, is needed for the activation of the endonucleolytic activity of DCLRE1C, probably by inducing a conformational change in PRKDC that provides DCLRE1C with access to DNA ends (Goodarzi et al. 2006, Niewolik et al. 2006, Jiang et al. 2015).

Literature References

PubMed ID	Title	Journal	Year
25818648	Differential Phosphorylation of DNA-PKcs Regulates the Interplay between End-Processing and End-Ligation during Nonhomologous End-Joining Zha, S, Crowe, JL, Dubois, RL, Nakajima, S, Lan, L, Liu, C, Yu, X, Liu, X, Li, C, Wang, Y, Lee, BJ, Jiang, W	Mol. Cell	2015
16874298	DNA-PK autophosphorylation facilitates Artemis endonuclease activity Ye, R, Härer, C, Walker, SA, Douglas, P, Marchetti, C, Lees-Miller, SP, Jeggo, PA, Goodarzi, AA, Morrice, N, Riballo, E, Yu, Y	EMBO J.	2006
16093244	The DNA-dependent protein kinase catalytic subunit phosphorylation sites in human Artemis Lieber, MR, Schwarz, K, Lu, H, Pannicke, U, Niewolik, D, Ma, Y	J. Biol. Chem.	2005
16914548	DNA-PKcs dependence of Artemis endonucleolytic activity, differences between hairpins and 5' or 3' overhangs Kulesza, P, Schwarz, K, Lieber, MR, Lu, H, Wang, LC, Pannicke, U, Zandi, E, Niewolik, D, Ma, Y	J. Biol. Chem.	2006
11955432	Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Schwarz, K, Pannicke, U, Lieber, MR, Ma, Y	Cell	2002